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NN414, a SUR1/Kir6.2-Selective Potassium Channel Opener, Reduces Blood Glucose and Improves Glucose Tolerance in the VDF Zucker Rat

¹ Department of Pharmacological Research I, Novo Nordisk, Bagsvaerd, Denmark
² Department of Medicinal Chemistry III, Novo Nordisk, Bagsvaerd, Denmark

	ABSTRACT

TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

 
A novel potassium channel opener compound, NN414, selective for the SUR1/Kir6.2 subtype of the ATP-sensitive potassium channel, was used to examine the effect of reducing ß-cell workload in the male Vancouver diabetic fatty (VDF) Zucker rat model of mild type 2 diabetes. Two chronic dosing protocols of NN414 of 3 weeks’ duration were compared with appropriate vehicle-treated controls. In the first group, rats received NN414 (continued group; 1.5 mg/kg p.o. twice daily), during which an oral glucose tolerance test (OGTT) (on day 19 of dosing) was performed and insulin secretion from an in situ perfused pancreas preparation (on day 21) was measured. The second group received NN414 (discontinued group; same dose), but active treatment was replaced by vehicle treatment 2 days before the OGTT and for a further 2 days before the perfused pancreas study. Basal glucose was significantly reduced by NN414, with the fall averaging 0.64 mmol/l after 3 weeks of treatment (P < 0.0001). The glucose excursion and hyperinsulinemia during the OGTT were significantly different between the continued, discontinued, and vehicle groups (glucose area under the curve [AUC]: 640 ± 29, 740 ± 27, and 954 ± 82 mmol · l^-1 · min^-1, respectively, P < 0.0001; insulin AUC: 38.9 ± 4.2, 44.2 ± 4.2, and 55.1 ± 2.6 nmol · l^-1 · min^-1, respectively, P < 0.0001). Hyperinsulinemia during the pancreas perfusion with 4.4 mmol/l glucose was significantly reduced in both treatment groups versus vehicle (P < 0.0005). Insulin secretory responsiveness to a step increase in glucose from 4.4 to 16.6 mmol/l, calculated relative to basal, was significantly improved in the continued group versus vehicle (P < 0.01). In conclusion, administration of NN414 for 3 weeks in VDF rats reduces basal hyperglycemia, improves glucose tolerance, and reduces hyperinsulinemia during an OGTT and improves insulin secretory responsiveness ex vivo. NN414 may therefore represent a novel approach to the prevention and treatment of impaired glucose tolerance and type 2 diabetes.

It has been known for >20 years that ATP-sensitive potassium channel openers (K_ATPCOs) can improve the insulin release pattern in human type 2 diabetes. This effect is thought to be mediated via hyperpolarization of ß-cells, thereby providing ß-cell rest by reducing insulin release. Thus, diazoxide has been shown to improve the release of insulin in response to intravenously administered glucagon and tolbutamide in type 2 diabetic patients (6). This early observation has more recently been confirmed by Guldstrand et al. (7), who demonstrated an improvement in first-phase insulin release in response to an arginine challenge in type 2 diabetic patients treated with diazoxide. Furthermore, improvement in the type 2 diabetes-induced insulin secretory defect has been observed in response to a hyperglycemic clamp after acute ß-cell rest induced by a somatostatin infusion (8). Moreover, there is evidence to support the contention that ß-cell rest may actually prevent the onset of diabetes. Thus, preclinically, it is known that diazoxide protects 90% pancreatectomized rats from impaired glucose-stimulated insulin release and increases pancreatic insulin content by 50% (9). Furthermore, diazoxide prevents sucrose-induced diabetes in rats (10). Together, these results suggest that inhibition of insulin release by use of K_ATPCO compounds will ameliorate insulin secretory capacity. However, it is still debatable for how long ß-cell rest has to be maintained to see improvements in insulin secretory capacity.

It remains uncertain whether the effects of diazoxide are mediated via the ß-cell or whether some other effect of the compound is responsible, because diazoxide is equally active on potassium channels incorporating the SUR1, SUR2A, and SUR2B subunits that are found in pancreatic, cardiac, and vascular tissues, respectively. Therefore, we used a novel orally active compound, selective for the SUR1/Kir6.2 potassium channel (11), NN414 [3-(1-methylcyclopropyl)amino-4H-thieno[3,2-e]-1,2,4-thiadiazine 1,1-dioxide], which is related pharmacologically to similar probe compounds, e.g., NNC 55-0118 (12,13), to study the specific effects of SUR1/Kir6.2 activation on the ß-cell in vivo.

	RESEARCH DESIGN AND METHODS

TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

 
Animals and drug administration regimen.
The VDF Zucker (fa/fa) rat strain has been established at the Department of Physiology, University of British Colombia (14). Animals are characterized by hyperinsulinemia and marked obesity but, unlike conventional Zucker obese rats, have mild diabetes. Age-matched (10–12 weeks old at the start of the dosing period) male obese animals (400–475 g) were used. Rats were initially allocated to groups of eight animals in each of four experimental groups (two treatment groups and two control groups), housed in standard 12-h light/12-h dark conditions, and given free access to food and water ad libitum.

Protocol.
NN414 was dissolved in vehicle (10% glycerol, 0.5% gelatin, and 10% cellulose in water) to give a stock solution of 1 mg/ml, which was prepared freshly every week. NN414 was administered orally in a dose volume of 1 ml/kg according to the following three dosing regimens:

Continuous dosing protocol.
Rats received NN414 at a dose of 1.5 mg/kg twice daily (8:00 A.M. and 5:00 P.M.) each day for 3 weeks. The dosing was continued until the 5:00 P.M. dose on day 18, and the oral glucose tolerance test (OGTT) was performed at 8:00 A.M. on day 19. Dosing was then resumed at 8:00 A.M. and 5:00 P.M. until day 20; the animals were killed before the in situ perfused pancreas study on the morning of day 21.

Discontinuous dosing protocol.
Rats received NN414 at a dose of 1.5 mg/kg twice daily (8:00 A.M. and 5:00 P.M.) each day for 3 weeks. The dosing was discontinued for 2 days before the OGTT, followed by a further 2 days before the day animals were killed for the isolated perfused pancreas study. Rats were dosed with vehicle only during the period of drug discontinuation.

Two vehicle-treated groups.
Rats received vehicle (1 ml/kg b.i.d.) at each time point corresponding to each of the treatment groups above. Data from these two groups were merged for the purposes of data analysis (control group).

Within each group, rats were dosed using a staggered daily dosing regimen that allowed each OGTT and isolated perfused pancreas study to be performed between 8:00 and 10:00 A.M.

Blood glucose, blood insulin, and body weight measurement.
Blood glucose was measured 5 min before each dose of NN414. Blood samples (2 x 50 µl) were obtained for insulin measurement by sampling from the tip of the tail. The insulin radioimmunoassay and its characterization have been described elsewhere (15). Body weight was measured daily 10 min before each dose.

OGTT.
Rats were fasted overnight, and on the morning of the study, samples were taken for determination of fasting blood glucose and insulin. An oral glucose challenge (1 g/kg) was administered to conscious unrestrained animals, and blood glucose was measured at 10, 20, 30, 60, and 120 min after glucose ingestion. Blood glucose was measured using a Surestep monitoring system (LifeScan, Burnaby, BC, Canada).

A composite insulin sensitivity index (CISI) was estimated from the OGTT, adapting the approach proposed by Matsuda and DeFronzo (16) and previously applied to VDF rats (17). CISI was calculated using the following equation:

where FBG is fasting blood glucose, FPI is fasting plasma insulin, and MG and MI represent the mean glucose and mean insulin concentrations during the course of the OGTT (area under the curve [AUC]/60 min). The factor 10,000 represents an arbitrary scaling constant.

Isolated perfused pancreas.
Animals were fasted overnight with the last dose of NN414 administered at 5:00 P.M. the previous evening. Pancreas isolation is described in detail elsewhere (15). Briefly, rats were anesthetized (60 mg/kg pentobarbital intraperitoneally) and the pancreas and associated duodenum were isolated. The perfusate was a modified Krebs-Ringer buffer containing 3% dextran (Sigma) and 0.2% radioimmunoassay grade BSA gassed with 95% oxygen and 5% carbon dioxide at pH 7.4, and supplied at a flow rate of 4 ml/min. First, a 10-min equilibration period with glucose concentration at 4.4 mmol/l was established, following which samples of emergent perfusate were collected at 1-min intervals for 6 min. The glucose concentration in the perfusate was then stepped up to 16.6 mmol/l for the next 34 min, and samples were collected every 30 s for the first 10 min of high glucose (for a more complete analysis of the first-phase insulin secretion) and every 1 min for the remainder of the perfusion period.

Statistical analysis.
Statistic analyses were performed with the SAS system for Windows, release 8.00. AUC was calculated using the trapezoidal rule. Means and SD were calculated. Statistical analysis of CISI was performed on reciprocal data (1/CISI) to obtain equal variances between groups. Student’s unpaired t test, paired t test, or ANOVA with Tukey’s studentized range test for post hoc pairwise comparisons were used as indicated. P < 0.05 was taken to indicate statistical significance.

	RESULTS

TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

 
Effect of NN414 on blood glucose levels throughout the dosing period.
Blood glucose levels were similar in all four groups before initiation of treatment (NS by ANOVA). For the first 3 weeks, treatment was identical in the continued and discontinued NN414 groups, and, therefore, the analysis for parameters measured on day 21 was performed with the two treated groups merged and compared with the two vehicle groups combined. In the vehicle-treated animals, blood glucose was stable with no difference between day 1 and 21 (0.01 ± 0.22 mmol/l, P = 0.83, paired t test). Conversely, in the 16 NN414-treated animals, glucose decreased progressively, with the difference between blood glucose on day 1 and 21 being significant (-0.63 ± 0.48 mmol/l, P < 0.0002, paired t test). Compared with the control group, NN414 significantly reduced blood glucose, with the average reduction amounting to 0.64 mmol/l by day 21 (P < 0.0001, unpaired t test). The basal blood glucose data are illustrated in Fig. 1.

View larger version (23K): [in this window] [in a new window]   FIG. 1. Blood glucose levels obtained during treatment with either NN414 (1.5 mg · kg-1 · day-1 b.i.d. for 3 weeks; •) or vehicle in VDF rats (). Data from both the continuous and discontinuous dosing regimens were combined and shown as the treated group. Data are means ± SD.

View larger version (25K): [in this window] [in a new window]   FIG. 2. Body weight measured during treatment with either NN414 (1.5 mg · kg-1 · day-1 b.i.d. for 3 weeks •) or vehicle in VDF rats (). Data from both the continuous and discontinuous dosing regimens were combined and shown as the treated group. Data are means ± SD.

View larger version (17K): [in this window] [in a new window]   FIG. 3. Ameliorative effect of NN414 (1.5 mg · kg-1 · day-1 b.i.d. for 3 weeks) on oral glucose tolerance (A) and insulin levels during OGTT (B) after both continuous and discontinuous dosing regimens. Data from vehicle-treated rats (), discontinued NN414-treated rats (), and continued NN414-treated rats () are shown. Data are mean ± SD.

Effect of NN414 on CISI.
Insulin sensitivity judged by the CISI was significantly improved in both treated groups (continued, 2.23 ± 0.27; discontinued, 1.63 ± 0.18; control, 1.02 ± 0.06 [arbitrary units]; P < 0.0001 by ANOVA).

Effect of NN414 on the pattern of insulin release from the isolated perfused pancreas preparation.
Treatment with NN414 significantly lowered the basal level of insulin release from the isolated perfused pancreas. Thus, the AUCs for perfusate insulin concentration were 20.9 ± 3.6, 24.2 ± 7.2, and 54.1 ± 26.5 nmol · l^-1 · min^-1 in the continued and discontinued treatment groups and the controls, with differences being statistically significant (P < 0.0005 by ANOVA, P < 0.005 continued vs. controls, P < 0.01 discontinued vs. controls). The glucose-dependent first-phase insulin release was assessed by expressing each data set as percent of basal, defined as the mean insulin concentration from each pancreas during the first 6-min perfusion period after the equilibration period. Thereafter, the AUC from 6 to 16 min (the predefined period during which insulin was measured every 30 s to assess first-phase release) was calculated. This normalized AUC is a measure of the insulin secretory responsiveness to the given glucose concentration change. First-phase insulin secretion defined in this way was 2,161 ± 361, 1,684 ± 431, and 1,435 ± 554%xmin in the continued and discontinued treatment groups and the controls, respectively (P < 0.01 by ANOVA, P < 0.01 continued vs. controls). Perfused pancreas data are shown in Fig. 4.

View larger version (27K): [in this window] [in a new window]   FIG. 4. Potentiation of first-phase insulin secretion from isolated perfused pancreas from either vehicle- or NN414-pretreated rats (1.5 mg · kg-1 · day-1 b.i.d. for 3 weeks) after both continuous and discontinuous dosing regimens. Data are means ± SD. A: Absolute insulin concentrations measured in perfusate fractions. B: Insulin secretion expressed relative to basal level during the first 6 min with the bar indicating the 30-s sampling period used to estimate first-phase insulin secretion. Glucose concentration in the perfusate was raised from 4.4 to 16.6 mmol/l after 6 min. SD bars have been omitted in B for clarity. The solid line shows the data from vehicle-treated rats, the long dashed line shows data from the discontinued NN414-treated rats, and the short-dashed line shows data from the continued NN414-treated rats.

	DISCUSSION

TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

The present study demonstrates that chronic treatment with NN414 results in a progressive antidiabetic effect and improves both the first-phase insulin release ex vivo and glucose tolerance in the mildly diabetic VDF rat strain. K_ATPCO-induced ß-cell rest may therefore have an effect on restoring the capacity of the ß-cell to produce an adequate first-phase insulin release in response to glucose.

The use of NN414 as a pharmacological probe for inducing ß-cell rest, commensurate with protracted use in vivo, is supported by a preliminary report showing tolerability and selectivity for suppression of insulin secretion in type 2 diabetic patients (20). It is probable that NN414 has direct effects on the ß-cell because the compound has been designed to be active selectively at the SUR1/Kir6.2 subtype of the potassium channels that are present on pancreatic ß-cells (11). However, we should bear in mind that SUR1 subunits are found at additional sites, meaning that SUR1/Kir6.2 selectivity does not necessarily confer ß-cell selectivity. For example, SUR1/Kir6.2 channels are also found in -cells, and, in unpublished studies, we have observed that a dose-dependent suppression of glucagon release by NN414 can be observed in isolated rat pancreas. The beneficial effects of NN414 reported here may therefore have been due to its interaction with pancreatic cells of different types and, indeed, nonpancreatic SUR1/Kir6.2 channels.

Most studies (9,21,22) looking at the beneficial effects of diazoxide in vivo have been conducted 2 or more days after cessation of an extended dosing period (equivalent to the discontinuous protocol in the present study). However, it remains unknown whether K_ATPCO therapy also has beneficial effects during the actual dosing period. For this reason, we included a group where the responses to the K_ATPCO were studied during the course of the dosing cycle (continuous dosing protocol) to investigate whether benefits of K_ATPCO therapy could also be seen under these conditions. Proof that benefits of pharmacologically induced ß-cell rest exist during the dosing period, in addition to the period after cessation of dosing, is important when considering the clinical potential of K_ATPCO therapy.

The observation that fasting blood glucose and plasma insulin are lower in both NN414-treated rats and control-treated rats suggests an improvement in glucose tolerance—an observation confirmed by an improvement in OGTT. This ameliorative effect of K_ATPCO drugs on glucose tolerance has also been demonstrated with the nonselective K_ATPCO, diazoxide, when administered chronically. Diazoxide (100 mg · kg^-1 · day^-1 for 8 weeks) reduced postabsorptive insulin levels and body weight gain in hyperinsulinemic obese Zucker rats compared with pair-fed controls, despite the fact that food intake increased slightly (22). Glucose tolerance was improved and postabsorptive glucose concentrations were lowered by the chronic diazoxide treatment. Leahy et al. (9) reported that glucose tolerance was improved in 90% pancreatectomized rats treated with diazoxide (30 mg/kg p.o. twice daily for 5 days), whereas Aizawa et al. (21) have shown that chronic administration of diazoxide (0.2% in diet) for 8 weeks to prediabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats prevents the development of insulin resistance and obesity in this model. In all of these studies, diazoxide treatment was terminated for 2 days before measurement of the beneficial effects of the compound.

A further aspect of the control of PIR by the pancreas is the question of whether an improvement in the insulin release pattern per se might be part of the explanation for the long-term effect of the compound on insulin sensitivity. We therefore studied the effects of the K_ATPCO on first-phase insulin release to investigate this possibility. An improved ß-cell responsiveness to glucose was observed in the perfused pancreas relative to baseline secretion. In absolute terms, however, no difference in pancreatic insulin output was observed between the groups during the step-up in glucose concentration, despite an apparent improvement in the continuously dosed group. Our interpretation that an improvement in first-phase insulin release exists as a result of NN414 treatment relies on the enhancement of insulin release relative to the basal release. However, in view of the hyperbolic relationship that exists between insulin secretion and insulin action (23), it is reasonable to interpret the difference in percent change as an improved ß-cell responsiveness viewed in relation to the prevailing degree of insulin resistance. This observation is in agreement with the hypothesis that the effect of K_ATPCO treatment on OGTT and first-phase insulin release contributes to the antidiabetic effect seen in these studies with NN414 and in the published studies with diazoxide. The relationship between ß-cell rest and resultant improvements in insulin secretory patterns has been studied in the clinic by use of simultaneous somatostatin infusion and hyperglycemic clamp and by observation of the effect of termination of the somatostatin infusion on resultant induced adverse insulin secretory patterns in type 2 diabetic patients (8). Somatostatin pretreatment improved the insulin secretory pattern compared with placebo-infused control subjects. We therefore speculate that the effect of the K_ATPCO drug might mimic the effects of somatostatin by causing ß-cell rest and consequential improvement in pancreatic function and that the amelioration of adverse glucose tolerance is secondary to long-term improvements in ß-cell function.

The progressive antidiabetic effect of NN414 on the mildly diabetic state of the VDF rat is suggestive that the long-term benefit of K_ATPCO drugs on the ß-cell is mediated via the SUR1/Kir6.2 channel, because the compound has been designed to act selectively at this subtype, and diazoxide, used in previous studies, does not act selectively at this subtype of channel.

Recent evidence is emerging that a discrete genetically determined increase in the open probability of SUR1/Kir6.2 may predispose affected people to develop type 2 diabetes (24,25) via a mechanism that reduces insulin release (26) and glucose-induced glucagon suppression (27). These observations suggest that constitutive activation of Kir6.2 could, at least in theory, predispose an individual to type 2 diabetes—an observation that might be viewed as somewhat contradictory with the present suggestion for a therapeutic application of K_ATPCO-opening agents. Our findings that a periodical opening of potassium channels improves ß-cell function during a meal do not necessarily contradict these observations in patients with permanently affected channels, suggesting that some periodicity with respect to potassium channel opening is an important determinant of the consequences on pancreatic function. Appropriate clinical studies, or studies involving genetically manipulated animals, may shed further light on this issue.

In conclusion, an antidiabetic effect of NN414 has been demonstrated both during and after cessation of K_ATPCO treatment in a strain of mildly diabetic rats. It is possible to speculate that ß-cell rest, induced pharmacologically, might interrupt a vicious circle of events whereby pancreatic overactivity culminates in progression and exacerbation of type 2 diabetes. We propose, therefore, that SUR1/Kir6.2-selective K_ATPCO drugs such as NN414 may have utility in the treatment (and prevention) of human type 2 diabetes.

	ACKNOWLEDGMENTS

 
The authors thank Professor Ray A. Pederson for providing access to the VDF rat colony, for giving valuable comments on the manuscript, and for providing practical and technical assistance.

	FOOTNOTES

 
All authors are employed by and hold stock in Novo Nordisk A/S.

Address correspondencereprint requests to Dr. Richard D. Carr, PhD, Pharmacological Research 1, Novo Nordisk A/S, DK-2880 Bagsvaerd, Denmark. E-mail: rdc{at}novonordisk.com

Received for publication February 10, 2003 and accepted in revised form June 23, 2003

Abbreviations: AUC, area under the curve; CISI, composite insulin sensitivity index; K_ATPCO, ATP-sensitive potassium channel opener; OGTT, oral glucose tolerance test; PIR, peripheral insulin resistance

	REFERENCES

TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

 

1. Hales CN, Randle PJ: Effects of low carbohydrate diet and diabetes mellitus on plasma concentrations of glucose, non-esterified fatty acid, and insulin during oral glucose-tolerance tests Lancet1 :790 –794,1963[Medline]
2. Ward WK, Bolgiano DC, McKnight B, Halter JB, Porte D: Diminished B cell secretory capacity in patients with non insulin-dependent diabetes mellitus. J Clin Invest74 :1318 –1328,1984
3. DeFronzo RA: Pathogenesis of type 2 diabetes: metabolic and molecular implications for identifying diabetes genes. Diabetes Rev5 :117 –269,1997
4. Leahy JL: Impaired beta cell function with chronic hyperglycaemia: ‘overworked beta cell hypothesis.’ Diabetes Rev4 :298 –319,1996
5. Polonsky KS, Sturis J, Bell GI: Non-insulin dependent diabetes mellitus: a genetically programmed failure of the beta cell to compensate for insulin resistance. N Engl J Med334 :777 –783,1996[Free Full Text]
6. Greenwood RH, Mahler RF, Hales CN: Improvement in insulin secretion in diabetes after diazoxide. Lancet1 :444 –447,1976[Medline]
7. Guldstrand M, Grill V, Björklund A, Lins PE, Adamson U: Improved beta cell function after short-term treatment with diazoxide in obese subjects with type 2 diabetes. Diabetes Metab28 :448 –456,2002[Medline]
8. Laedtke T, Kjems L, Pørksen N, Schmitz O, Veldhuis J, Butler PC: An overnight somatostatin-imposed inhibition of beta cell secretion restores orderliness and pulsatility of insulin secretion and the proinsulin to insulin ratio in patients with type 2 diabetes. Am J Physiol Endocrinol Metab279 :E520 –E528,2000[Abstract/Free Full Text]
9. Leahy JL, Bumbalo LM, Chen C: Diazoxide causes recovery of beta cell glucose responsiveness in 90% pancreatectomized rats. Diabetes43 :173 –179,1994[Abstract]
10. Gutman R, Basilico MZ, Mocchutti N, Chicco A, Lombardo YB: Diazoxide prevents the development of hormonal and metabolic abnormalities present in rats fed a sucrose rich diet. Horm Metab Res17 :491 –494,1985[Medline]
11. Wahl P, Hansen JB, Larsen T, Ashcroft FM, Dabrowski M: Potent and selective activation of beta-cell type ATP-sensitive potassium channels by NN414, a novel diazoxide analogue (Abstract). Diabetes51 (Suppl. 2) :A388 ,2002
12. Kullin M, Li Z, Hansen JB, Bjoerk E, Sandler S, Karlsson FA: KATP channel openers protect rat islets against the toxic effects of streptozotocin. Diabetes49 :1131 –1136,2000[Abstract]
13. Nielsen FE, Bodvarsdottir TB, Worsaae A, MacKay P, Stidsen CE, Boonen HCM, Pridal L, Arkhammar POG, Wahl P, Ynddal L, Junager F, Dragsted N, Tagmose TM, Mogensen JP, Koch A, Hansen JB: 6-Chloro-3-alkylamino-4H-thieno[3,2-e]-1,2,4-thiadiazine 1,1-dioxide derivatives potently and selectively activate ATP sensitive potassium channels of pancreatic beta cells J. Med Chem45 :4171 –4187,2002[Medline]
14. Lee S: Effects of diazoxide on insulin secretion and metabolic efficiency in the db/db mouse. Life Sci28 :1829 –1840,1981[Medline]
15. Pederson RA, Buchan AMJ, Zahedi-Asl S, Chan SB, Brown JC: Effect of jejunoileal bypass in the rat on the enteroinsular axis. Regul Pept5 :53 –63,1982[Medline]
16. Matsuda M, DeFronzo RA: Insulin sensitivity indices obtained from oral glucose tolerance testing. Diabetes Care22 :1462 –1470,1999[Abstract/Free Full Text]
17. Pospisilik JA, Stafford SG, Demuth H-U, McIntosh CHS, Pederson RA: Long-term treatment with dipeptidyl peptidase IV inhibitor improved hepatic and peripheral insulin sensitivity in the VDF rat. Diabetes51 :2677 –2683,2002[Abstract/Free Full Text]
18. Lopez N, Basabe J, Grant A, Krees S, Terry M, Viktora J, Wolff F: A044: An improved hyperglycaemic agent. Metabolism20 :373 –383,1971[Medline]
19. Lebrun P, Antione M-H, Ouedraogo R, Kane C, Dunne M, Hermann M, Herchuelz A, Masereel B, Delarge J, De Tullio P, Pirotte B: Activation of ATP-dependent K+ channels and inhibition of insulin release: effect of BPDZ 62. J Pharm Exp Ther277 :156 –162,1996[Abstract/Free Full Text]
20. Zdravkovic M, Kruse M, Rost KL, Jacobsen J, Møss J, Kecskes A: The safety, tolerability, pharmacokinetics and pharmacodynamics of NN414, a beta-cell type (SUR1/Kir6.2) potassium channel opener (KATPCO) following seven days once daily oral dosing in patients with type 2 diabetes (Abstract). Diabetes51 (Suppl. 1) :A117 ,2002
21. Aizawa T, Taguchi N, Sato Y, Nakabayashi T, Kobuchi H, Hidaka H, Nagasawa T, Ishihara F, Itoh N, Hashizume K: Prophylaxis of genetically determined diabetes by diazoxide: a study in a rat model of naturally occurring obese diabetes. J Pharm Exp Ther275 :194 –199,1995[Abstract/Free Full Text]
22. Alemzadeh R, Slonim AE, Zdanowicz MM, Matruo J: Modification of insulin resistance by diazoxide in obese Zucker rats. Endocrinology133 :705 –712,1993[Abstract]
23. Bergman RN, Phillips LS, Cobelli C: Physiologic evaluation of factors controlling glucose tolerance in man: measurement of insulin sensitivity and beta-cell glucose sensitivity from the response to intravenous glucose. J Clin Invest68 :1456 –1467,1981
24. Schwanstecher C, Meyer U, Schwanstecher M: K(IR)6.2 polymorphism predisposes to type 2 diabetes by inducing overactivity of pancreatic ß-cell ATP-sensitive K+ channels. Diabetes51 :875 –879,2002[Abstract/Free Full Text]
25. Schwanstecher C, Neugebauer B, Schulz M, Schwanstecher M: The common single nucleotide polymorphism E23K in K(IR)6.2 sensitizes pancreatic beta-cell ATP-sensitive potassium channels toward activation through nucleoside diphosphates. Diabetes51 :S363 –S367,2002[Abstract/Free Full Text]
26. Nielsen EMD, Hansen L, Carstensen B, Echwald SM, Drivsholm T, Glumer C, Thorsteinsson B, Borch-Johnsen K, Hansen T, Pedersen O: The E23K variant of Kir6.2 associates with impaired post-OGTT serum insulin response and increased risk of type 2 diabetes. Diabetes52 :573 –577,2003[Abstract/Free Full Text]
27. Tschritter O, Stumvoll M, Machicao F, Holzwarth M, Weisser M, Maerker E, Teigeler A, Haring H, Fritsche A: The prevalent Glu23Lys polymorphism in the potassium inward rectifier 6.2 (KIR6.2) gene is associated with impaired glucagon suppression in response to hyperglycemia. Diabetes51 :2854 –2860,2002[Abstract/Free Full Text]

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