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Polymorphism in the Calsequestrin 1 (CASQ1) Gene on Chromosome 1q21 Is Associated With Type 2 Diabetes in the Old Order Amish

¹ Division of Endocrinology, Diabetes and Nutrition, University of Maryland School of Medicine, Baltimore, Maryland
² Division of Endocrinology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
³ Geriatric Research and Education Clinical Center, Veterans Administration Medical Center, Baltimore, Maryland

	ABSTRACT

TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

 
Calsequestrin (CASQ)1 is involved in intracellular storage and release of calcium, a process that has been shown to mediate glucose transport in muscle. Its gene, CASQ1, is encoded on chromosome 1q21, a region that has been linked to type 2 diabetes in the Amish and several other populations. We screened all 11 exons, exon-intron junctions, and the proximal regulatory region of CASQ1 for mutations. We detected four novel single nucleotide polymorphisms (SNPs) (–1470CT, –1456delG, –1366insG, and 593CT). Ten informative SNPs within CASQ1 were genotyped in Amish subjects with type 2 diabetes (n = 145), impaired glucose tolerance (n = 148), and normal glucose tolerance (n = 358). Rs2275703 and rs617698 in introns 4 and 2 were significantly associated with type 2 diabetes (P = 0.008 and 0.04, respectively); three other SNPs showed borderline evidence for association to type 2 diabetes (P = 0.076–0.093). Furthermore, in nondiabetic subjects (n = 754), both rs2275703 and rs617698 were significantly associated with glucose area under the curve during an oral glucose tolerance test (P = 0.035 and 0.013, respectively). Haplotype analysis suggested that no haplotype could explain these associations better than rs2275703. These findings, coupled with similar findings in Utah Caucasians, suggest that sequence variation in CASQ1 may influence risk of type 2 diabetes.

We previously reported evidence for linkage of type 2 diabetes and impaired glucose homeostasis to a locus on chromosome 1q21-q24 (logarithm of odds 2.35, P = 0.0008) in the Old Order Amish (3). Linkage of this region on chromosome 1 to type 2 diabetes has been reported in at least five other populations, including Pima Indians (4), Utah Caucasians (5), French Caucasians (6), U.K. Caucasians (7), and Chinese (8); linkages of diabetes-related traits to this region have also been reported (9,10). Such robust replication provides strong evidence for a shared type 2 diabetes susceptibility locus in this chromosomal interval. However, this region contains at least 450 genes that include several excellent positional candidate genes, introducing the possibility that more than one gene variant in the region may influence diabetes risk. A number of positional candidate genes within this region have been studied to date, with variation in potassium inwardly rectifying channel, subfamily J, members 9 and 10 (KCNJ9/10) (22,23), liver-specific pyruvate kinase (PKLR) (24), C-reactive protein (CRP) (25), lamin A/C (LMNA) (26), and omentin (ITLN) (27) showing modest evidence for association with type 2 diabetes or related traits.

To further refine this interval with the aim of positionally cloning the type 2 diabetes gene(s) in this region, we embarked upon linkage disequilibrium (LD) mapping by performing genotype and association analysis of single nucleotide polymorphisms (SNPs) in Amish type 2 diabetic cases and nondiabetic control subjects. To date, 250 SNPs over the 28.5-Mb region from 148.5 to 170 Mb (NCBI [National Cancer for Biotechnology] build 34.3) have been genotyped. Multiple SNPs associated with type 2 diabetes were identified within the interval between 157 and 158.5 Mb, including rs617698, located within the calsequestrin 1 (CASQ1) gene. CASQ1 is a calcium storage protein localized in the sarcoplasmic reticulum of fast-twitch skeletal muscle cells. It is a constituent of the protein backbone of the luminal Ca²⁺ reservoir and plays a pivotal role in calcium flux through regulation of calcium channel activity and interaction with other proteins present in the sarcoplasmic reticulum (28). Calcium release from the sarcoplasmic reticulum into the cytosol has been shown to regulate GLUT4 expression (29) and glucose transport in muscle (30). Furthermore, Howarth et al. (31) reported that CASQ1 expression and calcium binding is increased in streptozotocin-induced diabetic rat skeletal muscle. We hypothesized that variation in CASQ1 may affect insulin action on glucose uptake and glycogen synthesis in skeletal muscle and thus type 2 diabetes susceptibility. To examine this positional candidate gene further, we screened CASQ1 for mutations and determined whether the observed sequence variation was associated with type 2 diabetes and related traits in the Old Order Amish.

	RESEARCH DESIGN AND METHODS

TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

 
The Old Order Amish are a genetically well-defined Caucasian founder population who live in a relatively homogeneous environment and have large sibships. Nearly all of these individuals share common ancestors; >95% of the chromosomal material of the Amish community of Lancaster County (now numbering 30,000 individuals) appears to have descended from <100 founders who emigrated to this area in the mid 1700s (12–14 generations) (32,33). The Amish Family Diabetes Study (AFDS) was begun in 1995 to identify susceptibility genes for type 2 diabetes and related traits. Probands with diabetes onset between 35 and 65 years and all willing first- and second-degree family members 18 years of age were invited to participate. DNA from 18 non–first-degree relatives with type 2 diabetes (36 alleles) from families providing evidence for linkage of diabetes to chromosome 1q21-q24 was used for sequence analysis of CASQ1. To define LD and haplotype structure of CASQ1, all SNPs were genotyped in a limited set of 258 Amish subjects chosen to be relatively unrelated (non–first-degree relatives) to each other. Next, to examine the relationship between haplotype-tagging polymorphisms in CASQ1 and diabetes, we genotyped DNA from subjects with type 2 diabetes (n = 145), impaired glucose tolerance (IGT) (n = 148), and normal glucose tolerance (NGT) (n = 358) for case-control association analysis. NGT subjects included in this analysis were required to be at least 38 years of age at the time of examination. For association analysis of quantitative traits (e.g., glucose and insulin), a larger set of 754 nondiabetic subjects (including the 506 NGT and IGT subjects described above) was studied. Informed consent was obtained from all AFDS participants, and the study protocol was approved by the institutional review board at the University of Maryland School of Medicine.

Trait measurements.
Details of methods for phenotyping of AFDS subjects have been previously described (3,34). Briefly, body height and weight were measured in all subjects using standard protocols. After a 12-h overnight fast, a 75-g oral glucose tolerance test (OGTT) was administered, with venous blood samples obtained at 0, 30, 60, 90, 120, 150, and 180 min for glucose and insulin measurements. The total glucose and insulin areas under the curve (AUCs) during the 3-h OGTT were calculated using the trapezoid method. Insulin resistance was estimated from fasting blood glucose and insulin levels with the homeostasis model assessment (HOMA) (HOMA of insulin resistance [HOMA-IR] = [fasting insulin {µU/ml} x fasting glucose {mmol/l}]/22.5). Diabetes was defined by fasting plasma glucose level (7 mmol/l), 2-h OGTT plasma glucose level (11.1 mmol/l), random plasma glucose level (11.1 mmol/l), the use of insulin or oral glucose-lowering agents, or a diagnosis of diabetes, with age of onset between 35 and 65 years, documented by a physician. IGT was diagnosed based on OGTT plasma glucose levels (2-h OGTT plasma glucose level between 7.8 and 11.1 mmol/l). NGT was defined based on fasting plasma glucose level (<6.1 mmol/l) and 2-h OGTT plasma glucose level (<7.8 mmol/l).

Mutation screening.
Primers were designed to specifically amplify CASQ1 exons, splice junctions, and 1,000 bp of the 5' flanking sequence using primer 3 (available at http//www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi) (see Table 1 of online appendix [available at http://diabetes.diabetesjournals.org]). PCR conditions were 95°C for 2 min, followed by 35 cycles of 95°C for 45 s, 56°C for 45 s, and 72°C for 1 min, with a final extension at 72°C for 7 min. Both strands were sequenced from DNA of the 18 subjects on an ABI 3700 DNA sequencer and analyzed with Sequence Analysis 3.2 software (Applied Biosystems Division, PerkinElmer, Foster City, CA).

Statistical analysis.
Before analysis, all SNPs were checked for Mendel errors using the PedCheck software program (37) in the extended Amish pedigree. A small number of Mendel errors were either resolved or genotypes removed before analysis. Allele frequencies of polymorphisms were determined by gene counting. Because of the extensive relatedness of study subjects in our Amish pedigrees, we tested for associations of SNP genotypes while accounting for the pedigree structure. Briefly, we used a variance component approach in which we modeled the probability that the subject was a case or control as a function of the individual’s age, sex, and genotype, conditional on the correlations in phenotype among relative pairs. For the primary analysis, we considered an additive genetic model in which the genotype was coded as 0, 1, or 2, depending on whether the subject was homozygous for the minor allele (genotype = 2), heterozygous (genotype = 1), or homozygous for the major allele (genotype = 0). Statistical testing was performed by testing the likelihood of the data under a model in which the genotype effect was estimated against the likelihood of a nested model in which the genotype effect was constrained to be zero. Under the likelihood ratio test, two times the difference in minus log₁₀ base likelihoods is distributed as a ² statistic with degrees of freedom equal to the difference in number of parameters between the models (in this instance, one). Secondary analyses were carried out assuming dominant and recessive genetic models, in which instance the genotype was coded as 0 or 1 depending on whether the subject carried the minor allele (genotype = 1 if minor allele carrier, 0 if not [dominant model]; genotype = 1 if homozygous for minor allele, 0 if not, [recessive model]). Parameter estimates (i.e., ß-coefficients) were obtained by maximum likelihood, and odds ratios (ORs) were obtained by taking the inverse log of the ß-coefficient. The OR for the additive model was scaled to reflect the odds that a case was homozygous for the minor allele versus the odds that the case was homozygous for the major allele. The variance components analysis was carried out using the SOLAR software program (38).

In similar fashion, we also evaluated the effect of genotype on levels of quantitative traits (e.g., glucose and insulin levels) by comparing mean trait levels across genotypes. We compared the likelihood of a model in which the trait values were allowed to vary by genotype (unconstrained model) with that in which the genotype effects were constrained to be zero. As before, the model likelihoods and parameter estimates were computed conditional on the pedigree structure, and likelihoods obtained from competing models were compared by likelihoods ratio test. Again, the additive model was used as our primary analysis, and dominant and recessive models were used as secondary analyses. The quantitative trait analyses were conducted in nondiabetic (NGT and IGT) individuals only.

The disequilibrium statistics r² and |D'| between each pair of SNPs were calculated using ZAPLO (39). For SNP pairs with r² > 0.9, only one of the two SNPs was genotyped in the full sample set. To determine the haplotype structure of SNPs with CASQ1, we used Haploview (40). We used Haploscore (41), which uses efficient score statistics within a general linear model framework, to test for haplotype associations between case and control subjects. Haploscore computes empirical P values by simulation. For all analyses, P values 0.05 were considered statistically significant, while P values between 0.05 and 0.1 were considered of borderline statistical significance.

	RESULTS

TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

 
Sequencing of all exons, exon-intron boundaries, and the proximal regulatory region of CASQ1 revealed a total of six polymorphisms. These include three novel SNPs upstream of the ATG start codon, –1470TC (CasqSNP1), –1456delG (CasqSNP2), and –1366insG (CasqSNP3), and one novel SNP in exon 3, 593CT (CasqSNP4), which was silent (Genbank accession no. NM_001231) (Fig. 1). In addition, we detected two SNPs previously reported in the dbSNP database (rs1186694 and rs3747623), located at 1,404 bp upstream of the ATG start codon and in intron 2, respectively. Of the 22 SNPs in and immediately flanking this gene reported in the public databases at the time of this study (including rs1186694 and rs3747623), we confirmed 11 by genotype analysis; the other 11 were not polymorphic (or had very low allele frequency) in the Amish (Fig. 1). In total, there were 15 SNPs in CASQ1; one occurred in the coding region and did not predict alterations in the amino acid sequence, four were in the 5' flanking region, one was in the 3' flanking region, and all others were in introns and did not predict any obvious alterations in RNA splicing.

View larger version (15K): [in this window] [in a new window]   FIG. 1. Schematic of CASQ1 and SNPs. Exons are shown as boxes and horizontal lines as introns, regulatory region or flanking segments. Novel SNPs discovered by sequence analysis are underlined, and dbSNPs are designated by the "Rs " numbers; those that were informative are above, while those that were not polymorphic in the Amish are below. SNPs with asterisks next to them were designated as informative haplotype-tagging polymorphisms and genotyped in the full sample set (see text for details). Note exon and intron sizes are not strictly drawn to scale.

We assessed the relationship between the SNPs and quantitative traits in an expanded set of 754 nondiabetic members of the AFDS. Three SNPs (rs617698, rs2275703, and rs822450) were significantly associated with glucose AUC during the OGTT (P < 0.05) (Fig. 2). These findings in nondiabetic subjects provide additional support for an effect of CASQ1 polymorphism on glucose homeostasis. There was no evidence for association of any of the SNPs with BMI, insulin levels (Fig. 2), or HOMA-IR.

View larger version (26K): [in this window] [in a new window]   FIG. 2. Association of CASQ1 SNPs rs617698 and rs2275703 with glucose and insulin levels during a 3-h OGTT in nondiabetic individuals. Both SNPs were associated with total OGTT glucose AUC (P = 0.013 and 0.035, addidtive model for rs617698 and rs2275703, respectively). The A allele of rs617698 appeared to better fit a recessive genetic model, with AA homozygotes having significantly higher glucose AUC than heterozygotes and GG homozygotes (P = 0.013). By contrast, the C allele of 2275703 appeared to better fit a dominant genetic model, with CC homozygotes and heteroyzgotes having higher glucose AUC than AA homozygotes (P = 0.03). *P < 0.05 between genotypes for a given time point. Rs822450 was also significantly associated with glucose AUC (P = 0.042) (data not shown). There was no evidence for association of rs617698 or rs2275703 with OGTT insulin levels.

View larger version (118K): [in this window] [in a new window]   FIG. 3. Haplotype structure of CASQ1 and flanking regions. Shown is the haplotype structure of CASQ1, as estimated by Haploview (40), on genotypes from 258 Amish subjects. |D'| values x100 are shown in the boxes, with empty boxes being 100 (or |D'| = 1). Not shown are CasqSNP1, which was in complete LD with CasqSNP2, and CasqSNP3 and rs2275705, which were both rare in the Amish (allele frequency 0.034 and 0.002, respectively). SNPs showing significant association to type 2 diabetes are underlined, while SNPs showing borderline significant association to type 2 diabetes are shown in italics.

	DISCUSSION

TOP ABSTRACT RESEARCH DESIGN AND METHODS RESULTS DISCUSSION REFERENCES

 
Chromosome 1q21-q24 has perhaps the best replicated linkage to type 2 diabetes in diverse populations, including Caucasians, Native Americans, and Chinese (3–10). Thus, there is a high likelihood that at least one type 2 diabetes susceptibility gene resides in this region. We and others have embarked upon a systematic LD mapping and positional candidate gene effort toward the positional cloning of the gene(s). Our LD mapping in Amish samples, as well as finer linkage analysis in Utah Caucasians (42), suggest at least two distinct type 2 diabetes loci within the 1q21-q24 interval. One of these regions, at 157–158.5 Mb, contains a number of good positional candidate genes, including CASQ1.

Calcium release from the sarcoplasmic reticulum into the cytosol has been shown to regulate GLUT4 expression (29) and glucose transport in muscle (30). Hayashi et al. (43) showed that an increase in cytoplasmic calcium is a key step in the activation of intracellular signaling cascades that mediate the immediate and prolonged effect of exercise on glucose transport. CASQ is present in muscle cells and located in the lumen of the sarcoplasmic reticulum. The major CASQ isoforms in skeletal (CASQ1) and cardiac (CASQ2) muscle exist as predominantly fast and slow isoforms, respectively (44). CASQ1 binds and releases large quantities of Ca²⁺ rapidly through its high capacity (40–50 Ca²⁺ per CASQ molecule) and relatively low-affinity interactions with Ca²⁺ (28,45). Howarth et al. (31) investigated the expression of CASQ and Ca²⁺ binding in cardiac and skeletal muscle from the streptozotocin-induced diabetic rat and found no significant changes in heart but an increase in the relative abundance of CASQ and CASQ-like proteins in skeletal muscle. Together, these data suggest that CASQ1 may play an important role in insulin- and/or contraction-stimulated glucose transport. We thus hypothesized that sequence variants in CASQ1 that alter expression or function may change intracellular Ca²⁺ flux and decrease glucose transport leading to glucose intolerance and/or diabetes.

We have extensively screened CASQ1 for variants in Amish subjects with type 2 diabetes. We identified four novel SNPs (three in the promoter and one in exon 3), none of which encode variants that would be expected to alter the protein’s structure. Although it is possible that the SNPs in the promoter may alter CASQ1 expression, none were associated with type 2 diabetes. Genotyping and association analysis of 10 informative polymorphisms revealed that two were significantly associated with type 2 diabetes and three others showed borderline evidence for association with type 2 diabetes. The two most significantly associated SNPs, rs2275703 and rs617698, are located in introns 4 and 2, respectively. Interestingly, the more common alleles of rs2275703 and rs617698 were the at-risk alleles, which is in line with the common disease, common variant hypothesis and with findings for other type 2 diabetes susceptibility genes, e.g., calpain 10 and Pro12Ala persoxisome proliferator–activated receptor 2. Supportive evidence that one or more of these variants influence glucose homeostasis is that allele frequencies of type 2 diabetes–associated SNPs were intermediate between type 2 diabetes and NGT in subjects with IGT (Table 1). Furthermore, rs2275703 and rs617698 were associated with glucose levels in nondiabetic subjects (Fig. 2).

Unfortunately, since our original linkage was obtained from large multiplex Amish families, determining how much of the original linkage can be accounted for by the CASQ1 SNPs is not straightforward. However, given the relatively modest evidence for association of CASQ1 SNPs with type 2 diabetes, it is unlikely that these SNPs (or haplotypes) can explain our original linkage result. Indeed, two-point linkage analysis with CASQ1 SNPs showed little evidence for linkage to type 2 diabetes or type 2 diabetes/IGT.

Might these associations represent false-positive results due to multiple comparisons? For association studies such as these, this is always a possibility. Indeed a full Bonferroni correction for multiple comparisons would render our associations nonsignificant (at the P < 0.05 level). However, we believe that correction for multiple comparisons is overly conservative, since some of our traits are correlated with each other. Furthermore, we have evidence for linkage in this region not only in the Amish but also in several other populations, which led to this hypothesis-driven LD mapping and positional candidate gene effort. Further reducing concern regarding false-positive results are two internal consistencies. First, the same SNPs showed association to type 2 diabetes and glucose traits in nondiabetic subjects. Second, for 8 of the 10 SNPs, the allele frequencies for the IGT cases were intermediate between those for the type 2 diabetes cases and the NGT control subjects.

Perhaps most compelling is that our findings are similar to studies conducted in an independent study of Utah Caucasians showing association of CASQ1 polymorphism with type 2 diabetes (46). The region of association with type 2 diabetes that overlaps in the Amish and the more outbred Utah Caucasian population appears to be within intron 2, specifically rs617599 and rs617598 in Utah Caucasians and rs617598 in the Amish (although this SNP was not the most significantly associated SNP in the Amish). Rs617599 and rs617598 are only 87 bp from one another and in moderate LD (d' = 0.82, r² = 0.62) in the Amish. However, contrary to our findings in the Amish, in which the rs617598 A allele was the at-risk allele for type 2 diabetes, the frequency of the G allele was significantly higher in Utah Caucasian subjects with type 2 diabetes. This discrepancy between the two populations may indicate that this SNP is not the functional SNP but is marking an at-risk haplotype that differs between the Amish and Utah Caucasians. Alternatively, these discrepancies between populations could represent false-positive or false-negative results.

The findings reported here do not provide insights into possible functional consequences of the variants in CASQ1. It is possible that variants in introns may affect mRNA splicing and/or expression. However, we cannot rule out the possibility that other variants in CASQ1 or other nearby genes in LD with CASQ1 SNPs might be the responsible functional variants.

We have observed evidence for association of other 1q21-q24 positional candidate genes with type 2 diabetes or related traits, namely lamin A (LMNA) (26), guanine exchange factor-11 (ARHGEF11) (M.S., A.R.S., unpublished observations), and omentin 2 (ITLN2) (27). While all of these genes reside under our linkage peak, they are 4.0, 3.2, and 0.75 Mb from CASQ1, respectively. There does not appear to be any significant LD for the two CASQ1 type 2 diabetes–associated SNPs, rs617598 and rs2275703, with SNPs in LMNA, ARHGEF11, or ITLN2, suggesting that the associations of variation in these genes with type 2 diabetes and related traits are independent of one another. These findings, coupled with more than one association signal in Pima Indians and Utah Caucasians, suggest that there are likely to be more than one gene (possibly several genes) responsible for the linkage at 1q21-q24.

In summary, we have reported a systematic search for polymorphism in CASQ1 on chromosome 1q21 and identified four novel SNPs. None predict alterations in the protein sequence. We describe for the first time a significant association between CASQ1 polymorphism and type 2 diabetes, possibly suggesting a new susceptibility gene for type 2 diabetes. Further analysis in other populations, as well as functional studies, will be necessary to further elucidate the role of polymorphism in CASQ1 in the pathogenesis of type 2 diabetes.

	ACKNOWLEDGMENTS

 
This work was supported by research grants R01-DK54261, K24-DK02673, U01 DK58026, and K07-CA67960; the University of Maryland General Clinical Research Center Grant M01 RR 16500; the General Clinical Research Centers Program, the National Center for Research Resources; the National Institutes of Health; and the Baltimore Veterans Administration Geriatric Research and Education Clinical Center.

We gratefully acknowledge our Amish liaisons and field workers and the extraordinary cooperation and support of the Amish community, without whom these studies would not have been possible.

	FOOTNOTES

 
Additional information for this article can be found in an online appendix at http://diabetes.diabetesjournals.org.

Address correspondence and reprint requests to Alan R. Shuldiner, MD, Division of Endocrinology, Diabetes and Nutrition, University of Maryland School of Medicine, 660 West Redwood St., Room 494, Baltimore, MD 21201. E-mail: ashuldin{at}medicine.umaryland.edu

Received for publication May 25, 2004 and accepted in revised form September 9, 2004

Abbreviations: AFDS, Amish Family Diabetes Study; AUC, area under the curve; CASQ, calsequestrin; HOMA, homeostasis model assessment; HOMA-IR, HOMA of insulin resistance; IGT, impaired glucose tolerance; LD, linkage disequilibrium; NGT, normal glucose tolerance; OGTT, oral glucose tolerance test; SNP, single nucleotide polymorphism

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