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Response to Comment on: Thallas-Bonke et al. (2008) Inhibition of NADPH Oxidase Prevents Advanced Glycation End Product–Mediated Damage in Diabetic Nephropathy Through a Protein Kinase C-–Dependent Pathway: Diabetes 57:460–469, 2008

¹ Juvenile Diabetes Research Foundation (JDRF) Albert Einstein Centre for Diabetes Complications, Diabetes and Metabolism Division, Baker Medical Research Institute, Melbourne, Victoria, Australia
² Department of Medicine and Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria, Australia

Corresponding author: Vicki Thallas-Bonke, JDRF Albert Einstein Centre for Diabetes Complications, Diabetes and Metabolism Division, Baker Medical Research Institute, P.O. Box 6492, St. Kilda Rd., Central, Melbourne, Victoria, Australia, 8008. Email: vicki.thallas{at}baker.edu.au

We thank Dr. Yamagishi for his constructive comments (1) on our recent article (2). In response, we agree that it is possible for protein kinase C– (PKC-) to be a downstream signaling molecule of NADPH oxidase, further validating this PKC isoform as an important therapeutic target for the treatment of progressive diabetic nephropathy. In the schema of Fig. 7 in our recent study, a question mark was attached to the arrow, suggesting that NADPH oxidase is downstream of PKC-. However, this issue remains to be fully clarified. Indeed, in our previous study (3), we reported that the renoprotective effects of treatments attenuating the accumulation of advanced glycation end products (AGEs), such as alagebrium, may in part occur via inhibition of PKC- activation. Dr. Yamagishi raises the possibility that PKC- activation may be a downstream event of the production of reactive oxygen species (ROS) via NADPH oxidase. We agree that this postulate cannot be excluded but is currently based primarily on in vitro studies.

We did not present any data in our study indicating that inhibition of PKC- suppresses in vivo superoxide generation and reduces levels of phosphorylated p47^phox. This relates to the lack of availability of a specific PKC- inhibitor for use in diabetic animal models and, therefore, we must rely on studies from the PKC- knockout model (4) to determine whether PKC- is an appropriate therapeutic target. It is likely that PKC- inhibition in diabetes would reduce albuminuria and renal vascular endothelial growth factor expression end points that were specifically examined in our study. Therefore, we agree that the overall theme of Dr. Yamagishi's letter emphasizing important bidirectional interactions among putative mediators of renal injury in diabetes is consistent with our own work, suggesting that combination therapies able to target multiple pathways are likely to ultimately be more successful than those targeting one specific molecule/pathway (5).

Received for publication March 24, 2008 and accepted in revised form March 28, 2008

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1. Yamagishi S: Comment on: Thallas-Bonke et al. (2008) Inhibition of NADPH oxidase prevents advanced glycation end product–mediated damage in diabetic nephropathy through a protein kinase C-–dependent pathway. Diabetes 57:e13, 2008. DOI: 10.2337/db08-0338[Free Full Text]
2. Thallas-Bonke V, Thorpe SR, Coughlan MT, Fukami K, Yap FY, Sourris KC, Penfold SA, Bach LA, Cooper ME, Forbes JM: Inhibition of NADPH oxidase prevents advanced glycation end product–mediated damage in diabetic nephropathy through a protein kinase C-–dependent pathway. Diabetes 57:460–469, 2008[Abstract/Free Full Text]
3. Thallas-Bonke V, Lindschau C, Rizkalla B, Bach LA, Boner G, Meier M, Haller H, Cooper ME, Forbes JM: Attenuation of extracellular matrix accumulation in diabetic nephropathy by the advanced glycation end product cross-link breaker ALT-711 via a protein kinase C-–dependent pathway. Diabetes 53:2921–2930, 2004[Abstract/Free Full Text]
4. Menne J, Park JK, Boehne M, Elger M, Lindschau C, Kirsch T, Meier M, Gueler F, Fiebeler A, Bahlmann FH, Leitges M, Haller H: Diminished loss of proteoglycans and lack of albuminuria in protein kinase C-–deficient diabetic mice. Diabetes 53:2101–2109, 2004[Abstract/Free Full Text]
5. Coughlan MT, Thallas-Bonke V, Pete J, Long DM, Gasser A, Tong DC, Arnstein M, Thorpe SR, Cooper ME, Forbes JM: Combination therapy with the advanced glycation end product cross-link breaker, alagebrium, and angiotensin converting enzyme inhibitors in diabetes: synergy or redundancy? Endocrinology 148:886–895, 2007[Abstract/Free Full Text]

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This Article Extract Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Request Permissions Google Scholar Articles by Thallas-Bonke, V. Articles by Forbes, J. M. PubMed Articles by Thallas-Bonke, V. Articles by Forbes, J. M. Social Bookmarking                What's this?