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Errata

Figure 1 of the above-listed article was printed too small and dark, making the figure hard to read. The enlarged figure is below.

View larger version (51K): [in this window] [in a new window]   FIG. 1. A: Representative Western blot showing a band migrating at 90 kDa corresponding to neprilysin protein. Mouse islet proteins were extracted, subjected to SDS-PAGE, immunoblotted with a polyclonal rabbit anti-neprilysin antibody, and then visualized by enhanced chemiluminescence. Mouse kidney was used as a positive control (right lane). A smaller band, likely nonspecific, was present in kidney but not islets. Each lane represents islet lysate from one female mouse. NT, nontransgenic; T, hIAPP transgenic. B: Representative islets from a hIAPP transgenic mouse stained for neprilysin (green), insulin (red), and nuclei (blue). Neprilysin appears to be localized to both ß-cells and non-ß-cells, as seen in the merge, and is membranous/cytoplasmic, as seen in the insert. In a subpopulation of islet cells, neprilysin staining is more intense (arrows). An identical pattern was seen in nontransgenic islets.

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