HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: db07-0617v1 57/4/817    most recent Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Li, Y. Articles by Wang, X. Search for Related Content PubMed PubMed Citation Articles by Li, Y. Articles by Wang, X. Social Bookmarking                What's this?

Homocysteine Upregulates Resistin Production From Adipocytes In Vivo and In Vitro

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University and the Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

Address correspondence and reprint requests to Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing 100083, China. E-mail: xwang{at}bjmu.edu.cn

Key Words: DMEM, Dulbecco’s modified Eagle’s medium • Hcy, homocysteine • HHcy, hyperhomocysteinemia • IKK, IB kinase • IL, interleukin • IRS, insulin receptor substrate • NAC, N-acetyl-cysteine • NF-B, nuclear factor-B • PAI, plasminogen-activator inhibitor • PKC, protein kinase C • ROS, reactive oxygen species • TNF, tumor necrosis factor

OBJECTIVE—Homocysteine (Hcy) is epidemiologically related to insulin resistance, which has been speculated to be a low-grade systemic inflammatory condition. Resistin acts as a critical mediator of insulin resistance associated with inflammatory conditions. We aimed to determine whether Hcy can induce insulin resistance by directly regulating the expression and secretion of resistin from adipose tissue.

RESEARCH DESIGN AND METHODS—The effect of Hcy on the expression and secretion of resistin and insulin resistance was investigated using primary rat adipocytes and mice with hyperhomocysteinemia (HHcy).

RESULTS—Hcy impaired glucose transport and, particularly, the insulin signaling pathway as shown by decreased insulin-stimulated tyrosine phosphorylation of insulin receptor and insulin receptor substrate (IRS)-1, increased serine phosphorylation of IRS-1, and inhibited Akt phosphorylation both in vitro and in vivo, and these impairments were accompanied by an increase in resistin expression. Compared with normal mice, HHcy mice with a clinically relevant level of plasma Hcy (19 µmol/l) showed significantly increased resistin production from adipose tissue (33.38 ± 3.08 vs. 19.27 ± 1.71 ng/ml, P < 0.01). Hcy (300–1000 µmol/l) also increased mRNA expression of resistin in primary rat adipocytes in a time- and concentration-dependent manner, with maximal induction at 24 h of approximately fourfold with 1,000 µmol/l. In addition, Hcy-induced resistin expression attenuated by treatment with reactive oxygen species (ROS) scavengers, protein kinase C (PKC), and nuclear factor (NF)-B inhibitors implies a role in the process for ROS, PKC, and NF-B.

CONCLUSIONS—HHcy may promote insulin resistance through the induction of resistin expression and secretion from adipocytes via the activation of the ROS-PKC–NF-B pathway.

CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?