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Diabetes Publish Ahead of Print published online ahead of print November 16, 2006
DOI: 10.2337/db06-1165
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Received for publication 17 August 2006
Accepted in revised form 6 October 2006

Islet-Derived Fibroblast-Like Cells Are Not Derived Via Epithelial-Mesenchymal Transition From Pdx-1 or Insulin-Positive Cells

Lucas G. Chase 1 , Fernando Ulloa-Montoya 1 , Benjamin L. Kidder 1 , and Catherine M. Verfaillie 1

From the 1 Stem Cell Institute, University of Minnesota, Minneapolis, Minnesota.

As recent studies suggest that newly formed pancreatic {beta}-cells are a result of self-duplication rather than stem cell differentiation, in vitro expansion of {beta}-cells presents a potential mechanism by which to increase available donor tissue for cell-based diabetes therapies. Although most studies have found that {beta}-cells are resilient to substantial in vitro expansion, recent studies have suggested that it is possible to expand these cells through a process referred to as epithelial-mesenchymal transition (EMT). To further substantiate such an expansion mechanism, we used recombination-based genetic lineage tracing to determine the origin of proliferating fibroblast-like cells from cultured pancreatic islets in vitro. We demonstrate, using two culture methods, that EMT does not underlie the appearance of fibroblast-like cells in mouse islet cultures but that fibroblast-like cells appear to represent mesenchymal stem cell (MSC)-like cells akin to MSCs isolated from bone marrow.


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