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Diabetes Publish Ahead of Print published online ahead of print March 16, 2007
DOI: 10.2337/db06-1398
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Original Research

Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle

Kirsten F. Howlett1, Kei Sakamoto3, Andrew Garnham1, David Cameron-Smith1, and Mark Hargreaves2

1School of Exercise and Nutrition Sciences, Deakin University, Burwood, 3125
2Department of Physiology, The University of Melbourne, Parkville, 3010, Australia
3MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, Scotland, United Kingdom

Correspondence: kirsten.howlett{at}deakin.edu.au

A single bout of aerobic exercise can enhance insulin action, but whether a similar effect occurs following resistance exericse is unknown. Hyperinsulinemic euglycemic clamps were performed on 8 male subjects at rest, and following a single, and 3 repeated bouts of resistance exercise over 7 days. Skeletal muscle biopsies were taken before and after the clamp, and immediately after a single exercise bout. Whole body insulin action measured by glucose infusion rate decreased (P<0.05) following a single exercise bout, whilst in response to repeated bouts of resistance exercise the glucose infusion rate was similar to the Rest trial. In skeletal muscle, AS160 phosphorylation, an Akt substrate implicated in the regulation of GLUT4 translocation, and its interaction with 14-3-3 was decreased (P<0.05) only following a single exercise bout. Insulin increased (P<0.05) phosphorylation of AS160 and its interaction with 14-3-3, but the insulin response was not influenced by resistance exercise. Phosphorylation of insulin receptor substrate-1 and Akt were similar to changes in AS160 phosphorylation following exercise and/or insulin. In conclusion, a single bout of resistance exercise impairs whole body insulin action. Regulation of AS160 and interaction with 14-3-3 in skeletal muscle are influenced by resistance exercise and insulin, but do not fully explain the effect of resistance exercise on whole body insulin action.


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Copyright © 2007 by the American Diabetes Association.