Diabetes
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Diabetes Publish Ahead of Print published online ahead of print June 27, 2007
DOI: 10.2337/db07-0502
This Article
Right arrow Full Text (PDF)
Right arrow
Right arrow Online-Only Appendix
Right arrow All Versions of this Article:
db07-0502v1
56/9/2251    most recent
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, R.
Right arrow Articles by Vasu, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, R.
Right arrow Articles by Vasu, C.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Original Research

Bone Marrow is a Preferential Homing site for Autoreactive T cells in type 1 diabetes

Ruobing Li, Nicolas Perez, Subha Karumuthil-Melethil, and Chenthamarakshan Vasu

Department of Surgery, University of Illinois at Chicago, Chicago, IL-60612, USA

Correspondence: chenta{at}uic.edu

Key Words: Type 1 diabetes • Autoimmunity • T cells • Bone marrow.

Objective:Pancreatic microenvironment is considered as the primary location of autoreactive T cells in type 1 diabetes. Diabetogenic T cells have also been detected in the spleen of NOD mice. However, it is not known whether the BM also contains T cells specific for self-antigen in hosts with autoimmunity. In this study, we investigated whether autoreactive diabetogenic T cells are present in the BM of NOD mice.

Research Design and Methods:BM and spleen T cells of female NOD mice were purified and tested for their cytokine secretion and proliferation in response to stimulation with immuno-dominant peptides of pancreatic ß cells. The diabetogenic nature and homing properties of purified BM T cells were compared with that of spleen T cells in NOD-Scid and wild-type mice.

Results:The BM T cells from both hyperglycemic and young euglycemic mice demonstrated profoundly higher proliferation and cytokine production in response to stimulation with ß-cell antigens as compared to T cells from spleen. The BM T cells showed rapid expansion and aggressive infiltration into pancreatic islets in NOD-Scid mice and induced hyperglycemia earlier than that observed with splenic T cells. Adoptive transfer of BM T cells resulted in their trafficking predominantly into BM and pancreatic LNs.

Conclusion:Our study shows that a large number of diabetogenic T cells are present in the BM of female NOD mice and these autoreactive T cells can be detected much before the clinical onset of the disease.


Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 2007 by the American Diabetes Association.