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The contribution of splanchnic fat to VLDL-triglyceride is greater in insulin resistant than insulin sensitive men and women: studies in the postprandial state

¹Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, OX3 7LJ, UK

Correspondence: barbara.fielding{at}oxlip.ox.ac.uk

Objective.: We aimed to determine differences in the postprandial contributions of different fatty acid sources to VLDL-TG in healthy men and women with varying degrees of insulin resistance.

Research Design and Methods.: Insulin resistant men (n=11), insulin sensitive men (n=11) and women (n=6), were given an intravenous infusion of [²H₂]palmitic acid to investigate systemic NEFA incorporation into VLDL-TG. Participants were also fed a mixed meal containing [U-¹³C]palmitic acid to investigate the contribution of dietary fatty acids to VLDL-TG production. Blood samples were taken over the following 6 h. Separation of VLDL was performed by density gradient ultracentrifugation and immunoaffinity techniques specific to apolipoprotein B-100.

Results.: Insulin resistant and insulin sensitive males had similar postprandial chylomicron and chylomicron remnant TG concentrations but insulin resistant males had higher postprandial VLDL-TG concentrations (AUC, 485 (123-992) vs. 287 (162-510) (median (range)) µmol/l, P<0.05). At 360 min, most of the difference in VLDL-TG was accounted for by an additional contribution from splanchnic fat (331±76 µmol/l vs. 89±25µmol/l, (mean ± SE) P<0.01). The contribution of fatty acids from endogenous systemic NEFA was similar across the groups, as were dietary fatty acids. There was no difference in the VLDL-TG concentration or the contribution of different fatty acid sources between insulin sensitive males and females.

Conclusions.: In the postprandial period, the only sources of fatty acids for VLDL-TG production to differ in the insulin resistant compared with the insulin sensitive males are those derived from splanchnic sources.

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