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Diabetes Publish Ahead of Print published online ahead of print February 14, 2008
DOI: 10.2337/db07-0722
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Original Research

Transgenic Mice Overexpressing Neuropeptide Y in Noradrenergic Neurons: A Novel Model of Increased Adiposity and Impaired Glucose Tolerance

Suvi T. Ruohonen, MS1,,4, Ullamari Pesonen, PhD1, Niko Moritz, PhD2, Katja Kaipio, MS1,,4, Matias Röyttä, MD, PhD3, Markku Koulu, MD, PhD1, and Eriika Savontaus, MD, PhD1,,5

From the 1Department of Pharmacology, Drug Development and Therapeutics
2Department of Orthopedics and Traumatology
3Department of Pathology and
4Drug Discovery Graduate School, University of Turku and
5Clinical Pharmacology, TYKSLAB, Health Care District of Southwest Finland, Finland

Objective: A functional polymorphism leucine 7 proline in the human neuropeptide Y (NPY) gene leading to increased NPY release from sympathetic nerves is associated with traits of metabolic syndrome. Although hypothalamic NPY neurons play an established role in promoting positive energy balance, the role of NPY co-localized with norepinephrine in sympathetic nervous system and brain noradrenergic neurons remains obscure.

Research Design and Methods: To clarify the role of NPY in noradrenergic neurons we generated a transgenic mouse overexpressing NPY under dopamine-beta-hydroxylase promoter and characterized the metabolic phenotype of the OE-NPYDβH mouse.

Results: NPY levels are increased by 1.3-fold in adrenal glands and 1.8-fold in the brainstem but not in the hypothalamus in OE-NPYDβH mice. They display increased white adipose tissue mass and cellularity, and liver triglyceride accumulation without hyperphagia or increased body weight. Hyperinsulinemia and impaired glucose tolerance develop by the age of six months in the OE-NPYDβH mice. Furthermore, circulating ghrelin is significantly increased in comparison with wildtype mice.

Conclusions: The present study shows that even a moderate increase in NPY levels in noradrenergic neurons leads to disturbances in glucose and lipid metabolism. The OE-NPYDβH mouse is an interesting new model to investigate the pathophysiology of some key components of the cluster of abnormalities characterizing the metabolic syndrome.

Correspondence: eriika.savontaus{at}utu.fi


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