Quantitation of Glycosylated Hemoglobin: Elimination of Labile Glycohemoglobin During Sample Hemolysis at pH 5

  1. Rudolf Flückiger
  1. Department of Research and Internal Medicine, University Clinics Kantonsspital, Basel, Switzerland
  1. Address reprint requests to Rudolf Flückiger, Zentrum für Lehre und Forschung, Diabetologie, Hebelstrasse 20, CH 4031 Basel, Switzerland.

Abstract

A simple method for the elimination of labile glycohemoglobin in the chromatographic quantitation of glycosylated hemoglobin is described. Use is made of the instability of Schiff base adducts in acidic solution. Erythrocytes are lysed with a pH 5 buffer. At this pH dissociation reaches completion during sample preparation.

  • Received March 17, 1982.
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