Assay for Islet Cell Antibodies: Protein A—Monoclonal Antibody Method

  1. G S Eisenbarth
  1. Joslin Diabetes Center, Brigham and Women's Hospital, and Harvard Medical School Boston, Massachusetts
  1. Address reprint requests to G. S. Eisenbarth, M.D., Ph.D., or S. Srikanta, M.D., Joslin Diabetes Center, Research Division, One Joslin Place, Boston, Massachusetts 02215.

Abstract

Assays for islet cell antibodies (ICA) are finding increasing application in clinical diabetology. We have developed a new islet cell antibody assay system (ICA-pA), whose salient features include: (1) utilization of fluorescein-conjugated staphylococcal protein A as a standard second-step reagent, the advantages of this approach being improved “signal” (islet)/“noise” (acini) ratio due to reduction of interfering background acinar pancreatic staining, and facilitation of assay standardization provided by the use of a chemically pure conjugated protein A reagent; (2) monoclonal antibody counterstaining with rhodamine-conjugated BISL-32 for the rapid identification of islets in pancreatic sections; and (3) quantitation of circulating serum ICA by microimmunofluorometric techniques.

  • Received December 24, 1984.
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