Glucagon-Like Peptide 1 Stimulates Lipolysis in Clonal Pancreatic β-Cells (HIT)
(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.
The effect of GLP-1 and forskolin on pHi in clonal pancreatic
β-cells. HIT cells (0.2 mg/ml) loaded with the pH indicator BCECF were
suspended in 1.3 ml Krebs buffer containing no albumin (pH 7.4) at 30°C.
Traces A and B show the acidification caused by the addition of GLP-1 or
forskolin. Trace C shows the alkalinization caused by glucose addition
followed by a small and transient acidification caused by GLP-1. The
illustrations shown are representative of experiments repeated at least three