Glucagon-Like Peptide 1 Stimulates Lipolysis in Clonal Pancreatic β-Cells (HIT)

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FIG. 3.
FIG. 3.

The effect of GLP-1 and glucose on FFA oxidation. Clonal β-cells (0.2 mg/ml) preloaded for 24 h with [U-14C]oleate were washed three times and preincubated in 1.3 ml Krebs buffer containing 5 mmol/l glutamine without glucose or FFAs (pH 7.4) at 30°C. After changing to a test medium containing no glucose, 10 mmol/l glucose, 10 nmol/l GLP-1, or GLP-1 plus 10 mmol/l glucose, 14CO2 evolution was determined during a period of 60 min. The data are means ± SE from six or eight flasks from two separate experiments. *P < 0.01 vs. basal or GLP-1 alone.

This Article

  1. Diabetes vol. 50 no. 1 56-62