Glucagon-Like Peptide 1 Stimulates Lipolysis in Clonal Pancreatic β-Cells (HIT)

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FIG. 5.
FIG. 5.

Triacsin C does not prevent glucose-mediated redox changes. HIT cells (0.2 mg/ml) were suspended in 1.3 ml Krebs buffer containing no albumin (pH 7.4) at 30°C. The top trace shows a decrease in oxidized flavin and the bottom trace shows an increase in reduced pyridine nucleotide fluorescence caused by the addition of glucose. Reduced pyridine (NADH) and oxidized flavin nucleotide (FAD) fluorescence were measured at excitation and emission wavelengths of 340 and 460 nm and 460 and 540 nm, respectively. The illustration shown is representative of experiments repeated at least three times.

This Article

  1. Diabetes vol. 50 no. 1 56-62