Glucagon-Like Peptide 1 Stimulates Lipolysis in Clonal Pancreatic β-Cells (HIT)
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Reverse transcription—PCR of rat pancreatic islet RNA (lane
I) using HSL-specific PCR primers. The products were separated on a 1.2%
agarose gel stained with ethidium bromide and photographed with UV
transillumination. The figure also shows the negative control lane (-) and the
molecular weight marker (MW). The band of the expected size was excised from
the gel, then the DNA was extracted and sequenced using Taq cycle sequencing
(with the original PCR primers) on an ABI 373 DNA sequencer.