Use of a cDNA Array for the Identification of Genes Induced in Islets of Suckling Rats by a High-Carbohydrate Nutritional Intervention
- Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York
Abstract
Four-day-old rat pups that are raised artificially on a high-carbohydrate (HC) milk formula immediately develop hyperinsulinemia, which persists into adulthood without any further nutritional stimulus. cDNA array analysis was used to identify large-scale changes in gene expression patterns in islets from 12- and 100-day-old HC rats in response to the HC dietary modification during the suckling period. It was observed that the expression of several genes that belong to clusters involved in β-cell development and/or β-cell function was significantly upregulated in islets from 12- and 100-day-old HC rats. It is inferred that in addition to predicted changes in gene expression, for example preproinsulin gene, global changes in gene expression contribute to the hyperinsulinemic state in the HC rat.
Footnotes
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Address correspondence and reprint requests to Mulchand S. Patel, Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, 140 Farber Hall, 3435 Main St., Buffalo, NY 14214. E-mail: mspatel{at}buffalo.edu.
Received for publication 6 June 2000 and accepted in revised form 16 May 2001.
ACC, acetyl CoA carboxylase; HC, high-carbohydrate; HMG, high-mobility group; IRS, insulin receptor substrate; Isl-1, islet factor-1; MF, mother-fed; PCNA, proliferating cell nuclear antigen, PDX-1, pancreatic duodenal transcription factor-1; REG, regenerating protein; RT-PCR, reverse transcriptase–polymerase chain reaction.
