Interleukin-6 Induces Cellular Insulin Resistance in Hepatocytes
- 1Graduate Program in Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, Rochester, New York
- 2Graduate Program in Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, New York
- 3Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York
Interleukin (IL)-6 is one of several proinflammatory cytokines that have been associated with insulin resistance and type 2 diabetes. A two- to threefold elevation of circulating IL-6 has been observed in these conditions. Nonetheless, little evidence supports a direct role for IL-6 in mediating insulin resistance. Here, we present data that IL-6 can inhibit insulin receptor (IR) signal transduction and insulin action in both primary mouse hepatocytes and the human hepatocarcinoma cell line, HepG2. This inhibition depends on duration of IL-6 exposure, with a maximum effect at 1–1.5 h of pretreatment with IL-6 in both HepG2 cells and primary hepatocytes. The IL-6 effect is characterized by a decreased tyrosine phosphorylation of IR substrate (IRS)-1 and decreased association of the p85 subunit of phosphatidylinositol 3-kinase with IRS-1 in response to physiologic insulin levels. In addition, insulin-dependent activation of Akt, important in mediating insulin’s downstream metabolic actions, is markedly inhibited by IL-6 treatment. Finally, a 1.5-h preincubation of primary hepatocytes with IL-6 inhibits insulin-induced glycogen synthesis by 75%. These data suggest that IL-6 plays a direct role in insulin resistance at the cellular level in both primary hepatocytes and HepG2 cell lines and may contribute to insulin resistance and type 2 diabetes.
Address correspondence and reprint requests to Robert A. Mooney, Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, 601 Elmwood Ave., Rochester, NY 14642. E-mail:.
Received for publication 28 March 2002 and accepted in revised form 9 September 2002.
DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; IL, interleukin; IκB, NF-κB inhibitor; IR, insulin receptor; IRS, IR substrate; NF, nuclear factor; PI, phosphatidlyinositol; SOCS, suppressors of cytokine signaling; TNF, tumor necrosis factor.