Variation in Three Single Nucleotide Polymorphisms in the Calpain-10 Gene Not Associated With Type 2 Diabetes in a Large Finnish Cohort
- Tasha E. Fingerlin12,
- Michael R. Erdos3,
- Richard M. Watanabe4,
- Kerry R Wiles3,
- Heather M. Stringham1,
- Karen L. Mohlke3,
- Kaisa Silander3,
- Timo T. Valle5,
- Thomas A. Buchanan6,
- Jaakko Tuomilehto5,
- Richard N. Bergman7,
- Michael Boehnke1 and
- Francis S. Collins3
- 1Department of Biostatistics, School of Public Health, University of Michigan, Ann Arbor, Michigan
- 2Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, Michigan
- 3Genome Technology Branch, National Human Genome Research Institute, Bethesda, Maryland
- 4Department of Preventive Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California
- 5Diabetes and Genetic Epidemiology Unit, Department of Epidemiology and Health Promotion, National Public Health Institute, Helsinki, Finland
- 6Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California
- 7Department of Physiology and Biophysics, Keck School of Medicine, University of Southern California, Los Angeles, California
Abstract
Variations in the calpain-10 gene have recently been reported to be associated with type 2 diabetes in a Mexican-American population. We typed three single nucleotide polymorphisms (SNPs) in the calpain-10 gene (SNPs 43, 56, and 63) to test for association between variation at these loci and type 2 diabetes and diabetes-related traits in 1,603 Finnish subjects: two samples of 526 (Finland-U.S. Investigation of NIDDM Genetics [FUSION] 1) and 255 (FUSION 2) index case subjects with type 2 diabetes, 185 and 414 unaffected spouses and offspring of FUSION 1 index case subjects or their affected siblings, and 223 elderly normal glucose-tolerant control subjects. We found no significant differences in allele, genotype, haplotype, or haplogenotype frequencies between index case subjects with diabetes and the elderly and spouse control populations (all P > 0.087). Although variation in these three SNPs was associated with variation in some type 2 diabetes-related traits within each of the case and control groups, no consistent pattern of the implicated variant or combination of variants was discerned. We conclude that variation in these three SNPs in the calpain-10 gene is unlikely to confer susceptibility to type 2 diabetes in this Finnish cohort.
Footnotes
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Address correspondence and reprint requests to Dr. Michael Boehnke, University of Michigan School of Public Health, Department of Biostatistics, 1420 Washington Heights, Ann Arbor, MI 48109-2029. E-mail: boehnke{at}umich.edu.
Received for publication 28 November 2001 and accepted in revised form 7 February 2002.
T.E.F. and M.R.E. contributed equally to this work.
AIR, acute insulin response; FUSION, Finland-U.S. Investigation of NIDDM Genetics; MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight; NPL, nonparametric linkage; OR, odds ratio; SI, insulin sensitivity; SNP, single nucleotide polymorphism.
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