Lipid-Induced Insulin Resistance in Human Muscle Is Associated With Changes in Diacylglycerol, Protein Kinase C, and IκB-α

TABLE 2

Distribution of PKC isoforms in the membrane and cytosolic fractions

Glycerol


Lipid


0 h −2 h −6 h 0 h −2 h −6 h
Membrane
 βI 0.03 ± 0.03 0.05 ± 0.02 0.086 ± 0.05 0.05 ± 0.02 0.17 ± 0.03 0.52 ± 0.3
 ε 0.27 ± 0.13 0.84 ± 0.75 0.16 ± 0.06 0.24 ± 0.11 0.55 ± 0.43 0.23 ± 0.05
 θ 0.44 ± 0.18 0.98 ± 0.86 0.14 ± 0.04 0.23 ± 0.11 0.45 ± 0.29 0.20 ± 0.05
 ζ 0.06 ± 0.01 0.07 ± 0.01 0.07 ± 0.01 0.10 ± 0.001 0.10 ± 0.01 0.11 ± 0.01
Cytosol
 βI 0.02 ± 0.02 0.04 ± 0.02 0.03 ± 0.01 0.06 ± 0.02 0.08 ± 0.02 0.07 ± 0.04
 ε 0.34 ± 0.19 0.21 ± 0.08 0.22 ± 0.09 0.25 ± 0.11 0.18 ± 0.08 0.02 ± 0.01
 θ 0.29 ± 0.11 0.14 ± 0.05 0.14 ± 0.06 0.15 ± 0.06 0.18 ± 0.06 0.13 ± 0.01
 ζ 0.03 ± 0.01 0.05 ± 0.01 0.05 ± 0.01 0.06 ± 0.003 0.04 ± 0.003 0.05 ± 0.001
  • Data are means ± SE. n = 6 for PKC-θ and ε, and n = 3 of densitometric units for PKC-βI and -ε. See legend to Figs. 2C and D for details

This Article

  1. Diabetes vol. 51 no. 7 2005-2011