Characterization of findings in APS−/− mice. A: Northern blot analysis for APS mRNA from various normal mouse tissues: adipose tissue, heart, brain, skeletal muscle, spleen,
lung, liver, and kidney. We loaded 2 μg poly (A)+ mRNA in each lane. B: Northern blot analysis for APS mRNA was done on adipose tissue and skeletal muscle to confirm the complete deletion of APS
in APS−/− mice. We loaded 2 μg poly (A)+ mRNA prepared from APS−/− or APS+/+ mice in each lane. C: APS protein expressions of liver, skeletal muscle, and adipose tissue were identified using Western blot analysis; 1 mg
crude protein extraction of each tissue prepared from APS−/− or APS+/+ mice was immunoprecipitated (IP) and immunoblotted (IB) using anti-APS COOH-terminus antibody (10), as described in research design and methods.