Increased Insulin Sensitivity and Hypoinsulinemia in APS Knockout Mice

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FIG. 4.
FIG. 4.

Effects of overexpression of APS on insulin-stimulated glucose uptake and GLUT4 translocation. A: Effects of exogenously overexpressed APS wild-type (WT) or Y618F mutant (YF) in 3T3L1-G4myc-CARΔ1 adipocytes on glucose uptake induced by insulin. These cells were prepared as described in research design and methods. Cells were incubated with (▪) or without (□) 100 nmol/l insulin for 10 min at 37°C. B: Effects of exogenously expressed APS wild-type or Y618F mutant in 3T3L1-G4myc-CARΔ1 on GLUT4 translocation induced by insulin (▪; 100 nmol/l insulin 10 min; □; buffer alone). Data of GLUT4myc translocation are expressed as fold stimulation relative to the extent of binding observed with cells that were not stimulated by insulin. The glucose uptake and GLUT4 translocation were measured as described in research design and methods. MOI; 10–20 pfu/cell. All values are expressed as the means ± SE.

This Article

  1. Diabetes vol. 52 no. 11 2657-2665