Increased plasma levels of leptin and adiponectin in APS−/− mice. A and B: Plasma concentrations of leptin and adiponectin in APS−/− mice. After a 14-h fast, blood samples of mice aged 8–11 weeks were collected from the inferior vena cava to measure plasma
leptin (A) and adiponectin (B) levels, respectively. APS+/+ (n = 23, means of body weight = 22 g) and APS−/− (n = 20, means of body weight = 23 g). All values are expressed as the means ± SD. *P < 0.05 vs. APS+/+ mice. C: Northern blot analysis for ob(leptin), Acrp30(adiponectin), APS, and β-actin mRNA in 3T3L1-G4myc-CARΔ1 adipocytes cells
overexpressed APS wild-type (WT), Y618F mutant (YF), or GFP as a control. Two micrograms of poly (A)+ mRNA prepared from 3T3L1-G4myc-CARΔ1 adipocytes overexpressed APS WT, YF mutant, or GFP by adenovirus infection, normal mouse
liver, or 3T3-L1 adipocytes with no adenovirus infection were loaded in each lane.