Enhanced Recombinant Adeno-Associated Virus-Mediated Vascular Endothelial Growth Factor Expression in the Adult Mouse Retina
A Potential Model for Diabetic Retinopathy
- P. Elizabeth Rakoczy1,
- Meliha Brankov2,
- Angela Fonceca1,
- Tammy Zaknich2,
- Ben C. Rae2 and
- Chooi-May Lai1
- 1Centre of Ophthalmology and Visual Science, the University of Western Australia, Nedlands, Australia
- 2Department of Molecular Ophthalmology, Lions Eye Institute of Western Australia, the University of Western Australia, Nedlands, Australia
Abstract
Diabetic retinopathy, one of the most serious complications of long-term diabetes, could clinically be divided into two stages: 1) background retinopathy that does not cause visual impairment and 2) proliferative retinopathy, which is a potentially blinding condition. This study aims to investigate the correlation between enhancement of vascular endothelial growth factor (VEGF) expression and neovascular changes. A binary recombinant adeno-associated virus construct producing green fluorescent protein (GFP) and VEGF under the control of the human cytomegalovirus promoter, recombinant adeno-associated virus (rAAV).VEGF.GFP, was produced and injected into the subretinal space of C57BL mice. GFP expression was tracked by fluorescence fundus photography, and VEGF expression was confirmed by immunohistochemistry and enzyme-linked immunoassay. Neovascular changes were monitored by fluorescein angiography and histology and by quantifying the number of inner retinal vessels. GFP expression was found in 100% of injected eyes, and vascular changes were detected in 9 of 10 rAAV.VEGF.GFP-injected eyes. Of these, four demonstrated microaneurysms and five showed moderate to severe leakage. There was a statistically significant increase in blood vessel number in the inner nuclear layer (P < 0.03) and dilatation of retinal veins (P ≤ 0.05). This work has demonstrated that the development of different stages of diabetic retinopathy is closely correlated with an increased VEGF level in the retina.
Footnotes
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Address correspondence and reprint requests to Professor P.E. Rakoczy, Centre for Ophthalmology and Visual Science, the University of Western Australia, 2 Verdun St., Nedlands 6009, WA, Australia. E-mail: rakoczy{at}cyllene.uwa.edu.au.
Received for publication 19 June 2002 and accepted in revised form 11 December 2002.
bFGF, basic fibroblast growth factor; ELISA, enzyme-linked immunosorbent assay; GCL, ganglion cell layer; GFP, green fluorescent protein; HRP, horseradish peroxidase; INL, inner nuclear layer; MMP, matrix metalloproteinase; PA, plasminogen activator; PCNA, proliferating cell nuclear antigen; rAAV, recombinant adeno-associated virus; RPE, retinal pigment epithelial; VEGF, vascular endothelial growth factor.
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