Glycation Inactivation of the Complement Regulatory Protein CD59
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Increased sensitivity to MAC-induced lysis and decreased hCD59 activity in diabetic RBCs. Diabetic RBCs (n = 12; HbA1c >8%) are much more sensitive than nondiabetic RBCs (n = 6; HbA1c <4%) to lysis induced by addition of purified terminal complement components to form the MAC (A) or by activating the classical pathway with anti-human RBC antibody and two different concentrations of human serum (15
and 30%; B). Inset: Diabetic RBCs have an 8–10 times lower CD59 activity than nondiabetic RBCs.