Role for Plasma Membrane-Related Ca2+-ATPase-1 (ATP2C1) in Pancreatic β-Cell Ca2+ Homeostasis Revealed by RNA Silencing

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FIG. 2.
FIG. 2.

Immunoblot of clonal β-cell fractions separated by differential centrifugation. INS1 (A) or MIN6 (B) cell fractions (20 μg per lane) were probed with rabbit anti-PMR1 (1:1,000) or, in A, with rabbit anti-mitochondrial glycerol phosphate dehydrogenase (mGPDH; 1:500; mitochondrial marker) or mouse anti-RyR II (1:1,000; ER marker). Immunoreactivity was visualized with anti-rabbit IgG HRP (1:80,000; Sigma) or anti-mouse IgG HRP (1:10,000; Sigma). INS1 fraction insulin content was measured by radioimmunoassay. Results shown are representative of three separate experiments performed for each cell type.

This Article

  1. Diabetes vol. 53 no. 2 393-400