Role for Plasma Membrane-Related Ca2+-ATPase-1 (ATP2C1) in Pancreatic β-Cell Ca2+ Homeostasis Revealed by RNA Silencing

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FIG. 3.
FIG. 3.

Silencing of endogenous PMR1 using siRNA. A: INS1 or MIN6 whole-cell lysates were prepared 0, 24, 48, and 72 h after transfection with control scrambled siRNA or PMR1 siRNA (as indicated). For each lane, 20 μg protein (or 10 μg where indicated) of siRNA-treated cell lysates were probed with rabbit anti-PMR1 (1:1,000) or, in B, with goat anti-SERCA2 (1:500; Santa Cruz) or rabbit anti-m6PR (1:1,000; Affinity BioReagents). Immunoreactivity was visualized with anti-rabbit IgG HRP (1:80,000; Sigma) or anti-goat HRP (1:10,000; Sigma). Each image is representative of three separate experiments for each cell type.

This Article

  1. Diabetes vol. 53 no. 2 393-400