Silencing of endogenous PMR1 using siRNA. A: INS1 or MIN6 whole-cell lysates were prepared 0, 24, 48, and 72 h after transfection with control scrambled siRNA or PMR1
siRNA (as indicated). For each lane, 20 μg protein (or 10 μg where indicated) of siRNA-treated cell lysates were probed with
rabbit anti-PMR1 (1:1,000) or, in B, with goat anti-SERCA2 (1:500; Santa Cruz) or rabbit anti-m6PR (1:1,000; Affinity BioReagents). Immunoreactivity was visualized
with anti-rabbit IgG HRP (1:80,000; Sigma) or anti-goat HRP (1:10,000; Sigma). Each image is representative of three separate
experiments for each cell type.