Role for Plasma Membrane-Related Ca2+-ATPase-1 (ATP2C1) in Pancreatic β-Cell Ca2+ Homeostasis Revealed by RNA Silencing

TABLE 1

Effects of PMR1 silencing on Ca2+ uptake and intracellular steady-state Ca2+ concentrations in intact cells

Compartment Initial rate of [Ca2+] rise (μmol · l−1 · s−1)
Peak [Ca2+] (μmol/l)
Control PMR1 Control PMR1
Cytosol 0.07 ± 0.03 0.44 ± 0.18* 1.25 ± 0.31 3.24 ± 1.00
Cytosol + nimodipine 0.07 ± 0.01 0.07 ± 0.01 1.65 ± 0.13 1.80 ± 0.22
Secretory vesicles 3.49 ± 0.53 3.41 ± 0.53 120.88 ± 2.12 125.40 ± 16.56
ER 3.57 ± 0.26 12.20 ± 2.12 142.99 ± 12.79 291.30 ± 45.23
ER + nimodipine 2.19 ± 0.25 2.23 ± 0.19 68.41 ± 6.55 56.61 ± 8.70
  • Control and PMR1 indicate data for cells treated with scrambled or PMR1 siRNAs, respectively.

  • *

    * Significance where P < 0.05;

  • P < 0.01.

This Article

  1. Diabetes vol. 53 no. 2 393-400