Regulation of ATP/ADP in Pancreatic Islets

  1. Ian R. Sweet1,
  2. Daniel L. Cook2,
  3. Eric DeJulio1,
  4. Angela R. Wallen1,
  5. Gamal Khalil3,
  6. James Callis3 and
  7. JoAnna Reems4
  1. 1Robert H. Williams Laboratory, Department of Medicine, University of Washington, Seattle, Washington
  2. 2Department of Physiology and Biophysics, University of Washington, Seattle, Washington
  3. 3Department of Chemistry, University of Washington, Seattle, Washington
  4. 4Puget Sound Blood Center, Seattle, Washington
  1. Address correspondence and reprint requests to Ian R. Sweet, HSB K-165, Box 357710, University of Washington, 1959 NE Pacific St., Seattle, WA 98195-7710. E-mail: isweet{at}u.washington.edu

Abstract

ATP and ADP levels are critical regulators of glucose-stimulated insulin secretion. In many aerobic cell types, the phosphorylation potential (ATP/ADP/Pi) is controlled by sensing mechanisms inherent in mitochondrial metabolism that feed back and induce compensatory changes in electron transport. To determine whether such regulation may contribute to stimulus-secretion coupling in islet cells, we used a recently developed flow culture system to continuously and noninvasively measure cytochrome c redox state and oxygen consumption as indexes of electron transport in perifused isolated rat islets. Increasing substrate availability by increasing glucose increased cytochrome c reduction and oxygen consumption, whereas increasing metabolic demand with glibenclamide increased oxygen consumption but not cytochrome c reduction. The data were analyzed using a kinetic model of the dual control of electron transport and oxygen consumption by substrate availability and energy demand, and ATP/ADP/Pi was estimated as a function of time. ATP/ADP/Pi increased in response to glucose and decreased in response to glibenclamide, consistent with what is known about the effects of these agents on energy state. Therefore, a simple model representing the hypothesized role of mitochondrial coupling in governing phosphorylation potential correctly predicted the directional changes in ATP/ADP/Pi. Thus, the data support the notion that mitochondrial-coupling mechanisms, by virtue of their role in establishing ATP and ADP levels, may play a role in mediating nutrient-stimulated insulin secretion. Our results also offer a new method for continuous noninvasive measures of islet cell phosphorylation potential, a critical metabolic variable that controls insulin secretion by ATP-sensitive K+–dependent and –independent mechanisms.

Free ATP and ADP levels are understood to couple glucose metabolism with the closing of ATP-sensitive K+ (KATP) channels and the ionic events leading to the exocytosis of insulin (1,2). Furthermore, the phosphorylation potential (ATP/ADP/Pi) may augment insulin secretion beyond that mediated by KATP channels (3). Although it is well established that the critical ATP and ADP levels depend on islet substrate metabolism and mitochondrial electron transport (46), it is less broadly appreciated that ADP stimulates electron transport and ATP production (7,8) as a feedback regulator of ATP/ADP/Pi (9,10). Thus, mitochondrial ATP production, the ATP/ADP/Pi, and ultimately the insulin secretory rate depend dually on both substrate supply as well as energy demand.

Although the mechanisms by which mitochondria sense ADP levels and control ATP/ADP/Pi are not fully established, a likely candidate is cytochrome c oxidase, the rate-limiting step in the electron transport chain (11). Wilson et al. (11,12) validated a mathematical model correlating substrate supply and energy demand (i.e., as phosphorylation potential) to the redox state and oxygen consumption rate of cytochrome c oxidase for several cell types, including cultured kidney cells (13), perfused rat heart (14), neuroblastoma cells (12), and heart mitochondria (11). To determine whether such dual control of electron transport exists in β-cells, we measured islet oxygen consumption and cytochrome c reduction in response to an increased and decreased energy state (glucose vs. cell activation by glibenclamide [15]) using a previously developed flow culture system (16,17). Our results support the dual control of electron transport in islet cells and suggest that changes of β-cell phosphorylation potential may be estimated noninvasively using the Wilson’s model (11).

RESEARCH DESIGN AND METHODS

Oxygen consumption and insulin secretion were measured using RPMI media 1640 (glucose-free; Gibco, Grand Island, NY) supplemented with 10% (vol/vol) dialyzed fetal bovine serum (FBS), antibiotic-antimycotic (100 units/ml penicillin, 100 μg/ml streptomycin, 0.25 μg/ml amphotericin B; all from Gibco), and glucose (Sigma, St. Louis, MO). For cytochrome c measurements, a phenol red-free minimum essential medium (MEM) was prepared (0.2 g/ml CaCl2, 98 mg/l MgSO4, 0.14 g/ml NaH2PO4H2O, 0.4 g/l KCl, 6.4 g/ml NaCl, 2.2 g/ml NaHCO3 without glucose [all from Sigma]; 20 ml/l essential amino acids solution, 10 ml/l nonessential amino acids solution, 10 ml/l MEM vitamin solution [all from Gibco]) supplemented with 10% (vol/vol) heat-inactivated FBS, 2 mmol/l glutamine, antibiotic-antimycotic, and varying amounts of glucose. Antimycin A and KCN were purchased from Sigma.

Islet isolation.

Rat islets were harvested from male Fisher rats (weighing ∼250 g), anesthetized by intraperitoneal injection of sodium pentobarbital (35 mg/230 g rat). Islets were prepared by injecting collagenase (10 ml of 0.23 mg/ml Liberase; Roche Molecular Biochemicals, Indianapolis, IN) into the pancreatic duct and surgically removing the pancreas. All procedures were approved by the Institutional Animal Care and Use Committee at the University of Washington. The pancreata were placed into 15-ml conical tubes containing 5 ml of 0.23 mg/ml Liberase and incubated at 37°C for 30 min. The digestate was filtered (400 μm stainless steel screen), rinsed (Hank’s buffered salt solution), and purified in a gradient solution of Optiprep (Nycomed, Oslo, Norway [18]). Islets were cultured for 18–24 h before the experiments in RPMI media 1640 supplemented with 10% heat inactivated FBS.

Flow culture system.

The islet flow culture system (16,17) includes a pump, gas equilibrator, a glass islet perifusion chamber, detectors for oxygen and cytochromes, and a fraction collector. To measure cytochromes by light absorption, islets were stabilized with Cytopore beads (Amersham Biosciences, Piscataway, NJ) that were layered into the chamber using a P200 pipette as follows (Fig. 1). First, 0.4 mg of beads in 20 μl media were allowed to settle onto the porous polyethylene frit at the chamber’s bottom. A mixture of 600 islets and Cytodex beads (0.12 mg; Amersham Biosciences) was added followed by another 0.4 mg Cytopore beads and a top frit. Porous frits were cored (0.3 cm) from polyethylene sheets (Small Parts, Miami Lakes, FL).

Oxygen consumption measurements.

Oxygen consumption was calculated as the flow rate multiplied by the difference between inflow and outflow oxygen tension, measured using phosphorescence lifetimes of an oxygen-sensitive polymerized platinum porphyrin (17). The dye, consisting of dimethylsiloxane-bisphenol A-polycarbonate blockcopolymer (5 g; GE, Waterford, NY) and platinum tetrapentafluorophenyl porphyrin (25 mg; Porphyrin Products, Logan, UT) dissolved in 100 ml dichloromethane, was painted inside the perifusion chamber 3 mm above the islets (Fig. 1) and excited by pulses from an ultraviolet light-emitting diode (395 nm; HUUV-5102; Roithner, Vienna, Austria). Decay lifetimes of emitted phosphorescent red light that correlate with oxygen tension were calculated as the ratio R = Ib/Ia, where Ib and Ia are the emission intensities 0–30 and 30–180 μs after the excitation pulse, respectively. The inflow oxygen tension was measured in the absence of islets in the chamber before and after each experiment, and for the calculations, oxygen content of media equilibrated with 5% carbon dioxide/balance air was assumed to be 217 nmol/ml.

Cytochrome measurements.

Cytochromes were measured by light transmission through the column of islets (17) using a Tungsten/Halogen 780 Lamp Supply (NRC; Newport, Irvine, CA), a spectrometer (Ocean Optics USB2000, Dunedin, FL), and 600-μm jacketed optical fibers. Integration times were 25–150 ms, and 55 signals were averaged per spectrum and ported into a laptop computer (Sony VAIO, Tokyo, Japan; Excel software, Microsoft, Redmond, WA). After each experiment, calibration spectra for fully oxidized and reduced cytochromes were acquired with 12 μg/ml antimycin followed by 3 mmol/l KCN.

Baseline drift is virtually eliminated (19) by using the second derivative of absorbance with respect to wavelength calculated as Formula

where Abs = log (intensity − intensitybkg)/(intensityref − intensitybkg), v = wavelength in nanometers, and Δ = change in the variable over the integration interval. Calculations were made using Excel (Microsoft, Redmond, WA). Background intensity (intensitybkg) was determined with the light source off, and the reference intensity (intensityref) was that obtained when cytochrome c was fully oxidized by antimycin A. Absorbance spectra were smoothed by a moving average in 10-nm intervals (15 points before and after a particular wavelength before calculating the second derivative). Percent reduction of cytochrome c (cytcred) was calculated following Kashiwagura et al. (20) as Formula

where Abs″ and Abs″KCN are values at 551 nm, and Abs″KCN is obtained in the presence of KCN when cytochrome c is fully reduced.

Insulin measurements.

Insulin was measured by enzyme-linked immunosorbent assay per the manufacturer’s instructions (ALPCO, Windham, NH).

RESULTS

Absorption spectra of rat islets.

Absorption by cytochrome c in islets was relatively small, but the second derivative revealed significant valleys at 551 nm (Fig. 2) that are most pronounced in the presence of cyanide, a blocker of cytochrome c oxidase subsequent to cytochrome a3 (Fig. 2A). The valley corresponding to cytochrome a3 (at 605 nm) was only detectable at a highly reduced state and could not be used in our system to assess the effects of glucose. Antimycin A, a blocker of electron transfer upstream of cytochrome c, fully oxidizes cytochrome c and the valley disappears (Fig. 2B). The magnitude of the valley at 551 nm was sensitive to glucose concentration (Fig. 2C and D), consistent with an increase in electron supply to the electron transfer chain. Thus, the state of cytochrome c made a major contribution to the absorption spectra of islets, and the depth of the valley at 551 nm reflected the extent of reduction.

Dynamic response of cytochrome c to glucose and oxygen.

Glucose stimulation (20 mmol/l) affected the cytochrome c redox state within 1 min (less than the temporal resolution of our system [Fig. 3A]) and required about 30 min to reach steady state (Fig. 3B). The kinetics of the increase and decay to baseline were nearly identical and demonstrated the precise relation between glucose and cytochrome c reduction. After 150 min at high glucose, the absorbance returned to baseline. Increasing media oxygen content from 20 to 95% only slightly altered the cytochrome c peak, suggesting that islets were well perifused by 20% oxygen and were not hypoxic. Equilibration with 0% oxygen dramatically reduced cytochrome c. We observed slow oscillations (period of ∼10 min) of cytochrome c reduction of an uncertain origin.

Absorbance data were normalized to spectra from cytochromes fully oxidized with antimycin A as a percentage of total cytochrome c (i.e., with KCN [Fig. 4]). The resulting ratio of [Cyt2+]/([Cyt2+] + [Cyt3+]), where [cyt2+] or [cyt3+] is the concentration of cytochrome C in the reduced oxidized state, is the fraction of total cytochrome in the reduced state and therefore represents a thermodynamic state variable that reflects one of two physiologically relevant driving forces of oxygen consumption.

Effect of glucose and glibenclamide.

Perifused islets were subjected to a 60-min pulse of either 20 mmol/l glucose or 1 μmol/l glibenclamide. As expected, increasing glucose increased cytochrome c reduction, oxygen consumption, and insulin secretion (Fig. 5), all of which decreased toward the baseline after stimulation. In Krebs-Ringer buffer (KRB), both the baseline rate of oxygen consumption and cytochrome c reduction were lower than that observed in RPMI. Oxygen consumption reached a plateau 45–60 min after the switch to high glucose, whereas cytochrome c response reached a new steady state after only 15 min. When islets were perifused with a salt solution (KRB) containing 10 mmol/l glucose, glibenclamide increased oxygen consumption (0.26 ± 0.11 to 0.37 ± 0.16 nmol · min−1 · 100 islets at peak−1) and produced a 17.5-fold increase in insulin secretion but did not affect cytochrome c reduction (Fig. 6B). In substrate-rich media, glibenclamide increased insulin secretion threefold but had no effect on either cytochrome c reduction or oxygen consumption (Fig. 6A).

Calculating phosphorylation potential.

Using the model derived by Wilson et al. (11) (see appendix, Eq. 3), ATP/ADP/Pi was calculated from the oxygen consumption and cytochrome c data (Figs. 5 and 6). High glucose reversibly increased the phosphorylation potential (Fig. 7A and C). Equation 4 predicts that, in the absence of changes in cytochrome c reduction, increased oxygen consumption should decrease phosphorylation potential. In KRB containing 10 mmol/l glucose, glibenclamide decreased the phosphorylation potential by 40% (Fig. 7D). In culture media where oxygen consumption was threefold higher than in KRB, glibenclamide changed neither cytochrome c reduction nor oxygen consumption; hence, phosphorylation potential was unchanged (Fig. 7B). Thus, a metabolizable substrate increased the energy state, whereas a nonmetabolizable secretogogue decreased the energy state, but only in KRB.

DISCUSSION

To understand how insulin secretion depends on cytoplasmic ATP and ADP by KATP-dependent and -independent pathways (3,4), we must understand how adenine nucleotide phosphorylation depends on substrate supply and metabolic demands. It is generally accepted that physiological increases in glucose concentration (5–10 mmol/l) increase ATP production and phosphorylation potential (4) that reflect a balance between ATP production and use (6). Mitochondria control this balance in aerobic cells (21), whereby oxygen consumption depends on both the supply of electrons to the electron transfer chain and changes in phosphorylation potential (10,11). Similar mechanisms may exist in islet cells because ADP stimulates mitochondrial respiration in β-HC9 cells (15), and Panten et al. (22) attributed glibenclamide-induced increased oxygen consumption to decreased phosphorylation potential. Our more precise measures of oxygen consumption and cytochrome c redox state provide additional evidence for the dual control of islet cell respiration, which suggests that ATP/ADP/Pi, the key metabolic variable controlling insulin secretion, can be estimated continuously and noninvasively using equations adapted from Wilson et al. (11).

Glucose increases cytochrome c reduction.

The reduction of cytochrome c increased robustly in response to glucose, reflecting an increase in the glucose-stimulated production of NADH. At 3 mmol/l glucose, the reduction of cytochrome c was less in KRB than in RPMI (28 vs. 40%; Fig. 5), possibly reflecting reduced substrate availability in KRB. However, at 20 mmol/l glucose, cytochrome c reduction was similar in KRB and RPMI (∼60%). The reduction at high glucose exceeded that reported for liver and kidney cells (21), possibly due to the unique effect of glucose to push the metabolic rate in islet cells. The tight relationship between glucose and cytochrome c is consistent with a mechanism involving transduction of the extracellular glucose signal by metabolism.

NADH and cytochrome c reduction are in equilibrium (2325) so that kinetic responses of NADH can be related to changes of cytochrome c reduction. However, standard measures of NADH autofluorescence at 460 nm are confounded by contributions from NADPH and other intracellular constituents in multiple compartments. In addition, fluorescence measures of either NAD(P)H or mitochondrial membrane potential yield only relative change, whereas our cytochrome c reduction method yields a calibrated signal that represents a thermodynamic driving force for oxygen consumption.

Kinetics of oxygen consumption in response to glucose.

Rates of oxygen consumption obtained depend on the method used. Whereas Cartesian divers record a nearly instantaneous response to glucose (26), continuous flow systems (22,27; the present study [Fig. 5]) record a slow (45- to 60-min) rise in oxygen consumption. To rule out an artifactual instrumentation delay, we determined that the oxygen sensor equilibrated in <10 min after an abrupt decrease in inflow oxygen tension; this must be an overestimate because metabolic poisons reduce islet oxygen consumption in <5 min (16). Thus, the gradual changes in oxygen consumption rate were observed 45–60 min after a glucose change. Although we cannot completely rule out baseline drifts after loading islets into our system, the characterization of our present system supports the validity of later more gradual changes in oxygen consumption and differences in the temporal response to glucose in KRB and RPMI.

Glibenclamide effects on oxygen consumption depend on media composition.

As in other studies of oxygen consumption (15,22), glibenclamide increased oxygen consumption in KRB but not in RPMI. We suggest two explanations for this. When islets were perifused with a substrate-poor buffer (KRB) containing 10 mmol/l glucose, oxygen consumption was ∼35% of that in substrate-rich RPMI media (Fig. 6), suggesting that glibenclamide-induced ATP consumption is a smaller fraction of the high ATP turnover seen in substrate-rich media. This would not be the case if glibenclamide also stimulates substrate usage as suggested (15). However, the inability of glibenclamide to enhance cytochrome c reduction or tolbutamide to change NADH levels (28,29) strongly argues against this. Because endoplasmic and membrane calcium transport consume ATP, differences in oxygen consumption may depend on the difference in KRB and RPMI calcium levels (2.6 vs. 0.42 mmol/l). Whatever its cellular effects, we simply used glibenclamide as a means to perturb and validate our model of the relationship between cytochrome c reduction, oxygen consumption, and phosphorylation potential.

Determining ATP/ADP/Pi.

It is important to recognize that despite the central importance of adenine nucleotides in mediating insulin secretion, direct measures of the thermodynamically relevant free ATP and ADP are not available (6,30). The most direct approach has been to rapidly freeze isolated islets and measure their total cellular ATP and ADP content. This provides good estimates of free ATP but not of free ADP because 95% of cellular ADP is bound or sequestered (30,31). Accordingly, only small increases in ATP/ADP have been seen in response to glucose (32,33). By identifying a large stable pool of nucleotides in islet secretory granules, an approximate fourfold increase in ATP/ADP was seen in response to glucose stimulation (34,35). However, even these estimates are ∼10-fold higher than estimates of free ADP level based on calculations that assume that the creatine kinase reaction that buffers ATP and ADP levels is in equilibrium (30,31).

Given the importance of free ATP and ADP to islet cells and the difficulty of measuring free ADP, we have suggested a new, albeit indirect, method for a continuous noninvasive measure of these key metabolic variables. Our analysis relies on the kinetic model derived by Wilson et al. (11) that describes oxygen consumption in terms of substrate pressure (cytochrome c reduction) and energy demand as reflected by ATP/ADP/Pi. The system includes electron transfer from NADH to oxygen via complexes I, III, and IV in the electron transport chain, and the creation of the transmembrane proton gradient that ultimately phosphorylates ADP by F0F1 ATPase. The complexity of these biochemical and biophysical processes was reduced according to three observations. First, the catalytic capacities of the electron transport chain reactions from NADH to cytochrome c (sites 1–3) are nearly at equilibrium (2325) and therefore are unlikely to regulate pathway flux. Similarly, the capacity of the F0F1 ATPases is sufficiently high that the conversion of biophysical energy in the proton gradient to chemical energy in ATP also operates at near-equilibrium. In contrast, the cytochrome c oxidase reaction that transfers electrons to O2 to produce H2O is nearly irreversible and not at equilibrium and thus is a candidate control site. Second, the flux of electrons pushes the cytochrome oxidase reaction farther from the equilibrium so that spectroscopic monitoring of the ratio of reduced to oxidized cytochrome c provides a noninvasive measure of this driving force. Third, Wilson’s steady-state (but nonequilibrium) kinetic model of the cytochrome oxidase reaction yields an algebraic expression (see appendix) that can predict the ATP/ADP/Pi ratio from the measured oxygen consumption rate and reductive state of cytochrome c.

Effect of glucose and glibenclamide on ATP/ADP/Pi.

Increasing glucose resulted in increased cytochrome c reduction and oxygen consumption (Fig. 5) and calculated ATP/ADP/Pi levels (Fig. 7). In contrast, a non-nutrient stimulator of insulin secretion, glibenclamide, increased oxygen consumption in islets perifused in KRB ∼50%, similarly to Panten et al. (22), but had little effect on cytochrome c reduction (Fig. 6A). Calculated ATP/ADP/Pi levels decreased in response to glibenclamide and presumably provided the driving force for the increased oxygen consumption. Thus, increased substrate pressure elicited by glucose resulted in an increased energy state, and an agent that induced the increased work of ion transport and insulin secretion in the absence of fuel decreased the energy state. Comparison of the magnitude of the calculated changes with direct measurements of phosphorylation potential are not possible because of the lack of a direct method to measure free ADP (6). However, 31P–nuclear magnetic resonance provides noninvasive estimates of phosphocreatine (PCr) and inorganic phosphate (Pi) in live cells (ADP levels are below the detection limit), and PCr/Pi is a measure of energy state that is correlated with the ATP/ADP/Pi. In studies of β-HC9 cells loaded with creatine, the ratio of PCr/Pi increased fourfold in response to glucose and decreased by 50% in response to glibenclamide (15), and studies measuring the effect of glibenclamide on total ATP/ADP in islets have reported 20–40% decreases (32,33). The directionality of the changes in energy state is consistent with the calculations in our study. The implication of this scenario is that phosphorylation potential is determined by substrate pressure and the mitochondrial coupling mechanisms rather than by the multifarious processes that use ATP.

Summary and conclusions.

We have combined measures of cytochrome c reduction and oxygen consumption to calculate changes in phosphorylation potential based on a kinetic model developed by Wilson et al.(11). Our results support the dual control of islet mitochondrial respiration by substrate availability and energy demand and offer a new method for continuous noninvasive measures of islet cell phosphorylation potential, a critical metabolic variable that controls insulin secretion by KATP-dependent and -independent mechanisms.

APPENDIX

Minimal model of coupled electron transport and oxidative phosphorylation based on a five-step mechanistic description of the kinetic properties of cytochrome c oxidase

Reduction of model complexity by focusing on rate-limiting steps.

Wilson et al. (11) derived a minimal model that describes oxygen consumption in terms of substrate pressure (cytochrome c reduction) and energy demand as reflected by ATP/ADP/Pi. The system transfers electrons from NADH to oxygen via complexes of the electron transport chain, the proton gradient, and ultimately the phosphorylation of ADP by F0F1 ATPase. However, the remarkable reduction in complexity of the model that was achieved was based on the experimental findings that the reactions in the electron transport chain from NADH to cytochrome c (sites 1–3) are in near-equilibrium (2325), where the overall equation is Formula Thus, phosphorylation of ADP, generation of proton gradients, and transport of adenine nucleotides across the mitochondrial membrane are fast, as is the transfer of electrons from NADH to cytochrome c, so these steps cannot be rate-controlling. The near-equilibrium status of Eq. 1 implies that respiratory control must reside in cytochrome oxidase as the rate-limiting step in oxygen consumption (11). Therefore the kinetics of coupled mitochondrial electron transport and oxidative phosphorylation can be represented by the factors controlling cytochrome oxidase. The thermodynamic driving force for cytochrome oxidase is determined by the reduced to oxidized ratio of any intermediates from NADH to cytochrome c. Because cytochrome c is the only intermediate where this ratio can be readily and noninvasively measure, the rate of oxygen consumption was modeled in a five-step mechanism describing the transfer of electrons from cytochrome c to oxygen via cytochrome oxidase.

The uncoupling effects of uncoupling protein 2, which may govern the levels of ATP/ADP/Pi (36,37), is not considered in this analysis. Neglecting to consider the dissipation of energy as heat will cause the prediction of ATP/ADP/Pi from the model to be overestimated. However, the fact that the directionality and order of magnitude of the changes in the phosphorylation potential measured in this study (see discussion) were similar to those found in other studies indicates that uncoupling protein 2 may modify but not override the basic mitochondrial coupling mechanisms and justifies this simplification for this initial test of the hypothesis in islets. It is also assumed that all oxygen consumption is due to respiration, which is supported by studies showing that inhibitors of electron transport resulted in a 70–100% inhibition of oxygen consumption (27).

Derivation of the mathematical model.

In Wilson’s reaction scheme (11), electrons are first transferred to a copper molecule that is bound to the cytochrome a3 subunit of cytochrome c oxidase (reaction 1). The reduction of the Cu atom “opens” the oxygen binding site, allowing oxygen to diffuse in and complex with the Cu (reaction 2). The oxygen complex can rearrange and bind to reduced cytochrome a3 (reaction 3). The two partially reduced states can accept one reducing equivalent from cytochrome c and then transfer two equivalents to the oxygen moiety to create bound-peroxide intermediate (reactions 4a and 4b). The cycle is completed by the acceptance of two more electrons and release by water (reaction 5).

The corresponding differential equations for the chemical reactions were solved for steady-state conditions, and an algebraic relation between oxygen consumption rate (vOCR), cytochrome c reduction, and phosphorylation potential was derived (Eq. 2, which is equivalent to Eqs. 3–5 in the study by Wilson et al. [11]). Consistent with classic observations of respiratory control, oxygen consumption is increased by both the supply of electrons traversing the electron transport chain as well as the demand for ATP mediated by decreased ATP/ADP/Pi levels. Formula where Formula Small k’s represent rate constants, and large K’s represent thermodynamic equilibrium constants of the reactions shown. a3T is the total concentration of cytochrome a3. The model, rewritten as a function determining ATP/ADP/Pi, becomes Formula where cT is the total concentration of cytochrome c and cytcred is the percentage of cytochrome c in the reduced state (see research design and methods). Here it is assumed that the magnitude of the second derivative of absorption is linearly proportional to the concentration of cytochromes such that cytcred equals 100 × [cyt c2+]/([cyt c2+] + [cyt c3+]). Values of rate and equilibrium constants used in the calculations in this article were those estimated from experiments on pigeon heart mitochondria (11) (Table 1), and a3T was assumed to be one-half of the value of cT, as was the case for pigeon heart mitochondria. A value of cT was not known a priori. Using a value for ATP/ADP/Pi (0.02) obtained in islets (30) and values for oxygen consumption rate and cytochrome c reduction, Eq. 3 was solved for cT to yield 3.5 μmol/l, which was used for all calculations.

FIG. 1.

Flow chamber housing the islets. Media equilibrated with gas entered the bottom of the chamber (2.5 mm internal diameter) and percolated up through the porous frit, continuously supplying the islets with fresh media. The total volume of the chamber was ∼200 μl. Oxygen consumption was measured by lifetime detection of an oxygen-sensitive phosphorescent dye that was painted on the interior of the glass chamber ∼1 cm above the islets. Measurement of cytochrome reduction was done by detecting the absorption of light by the islets in the chamber. Excitation and detection of light was transmitted to the sources and detection systems via optical fibers.

FIG. 2.

Effect of KCN, antimycin A, and glucose on absorption spectra. Representative spectra obtained from 600 rat islets perifused in MEM are shown. When cytochromes in the islets were fully reduced by the addition of cyanide, valleys centered around 551 nm for cytochrome c and 605 nm for cytochrome a3 were well resolved (A). In contrast, in the presence of antimycin A, a blocker of cytochrome b, electrons were prevented from transferring to cytochrome c and both valleys were not detectable (B). At low glucose, only the cytochrome c valley is detectable (C), which increases in depth at 20 mmol/l glucose (D). Each spectrum shown is the average of 55 acquisitions, each with an integration time of 50–150 ms.

FIG. 3.

Effect of glucose and oxygen on cytochrome c in rat islets (A and B). The concentration of glucose under normoxic conditions was switched between low and high glucose resulting in a reproducible increase and return to baseline of absorption due to cytochrome c. Pre-equilibrating the media with 95% oxygen only slightly decreased the absorption, indicating that islets were adequately oxygenated even when the media was normoxic. When the media was pre-equilibrated with 0% oxygen, the absorption increased dramatically reflecting the reduction of the cytochrome c. Spectra were obtained every minute, and the flow rate was 200 μl/min MEM.

FIG. 4.

Calibration of absorption data. After each experimental protocol, the effects of antimycin A (Anti A) and KCN were obtained to establish the fully oxidized and reduced state (A). Using the antimycin A spectra as a reference, absorption data were represented as the percent reduction relative to complete reduction established by the spectra obtained in the presence of cyanide (B). The percent reduction is defined in research design and methods.

FIG. 5.

Effect of glucose on oxygen consumption rate (OCR), cytochrome c reduction, and insulin secretion rate (ISR). Islets were perifused, during which glucose was increased from 3 to 20 mmol/l for 60 min and then returned to 3 mmol/l. A: For oxygen consumption and insulin secretion, the flow rate was 50 μl/min RPMI for 300 islets, and for cytochrome c measurements, the flow rate was 200 μl/min MEM for 600 islets. The increase in glucose elicited an increase in all three parameters. Each point represents the average of three separate experiments; the SE averaged for all data points for cytochrome c reduction was 3.4% and for oxygen consumption rate was 0.1 nmol · min−1 · 100 islets−1. B: In these experiments, cytochrome c reduction, oxygen consumption, and insulin secretion were measured simultaneously. The flow rate was 100 μl/min KRB for 600 islets. Each point represents the average of six separate experiments; the SE averaged for all data points for cytochrome c reduction was 5.4% and for oxygen consumption rate was 0.6 nmol · min−1 · 100 islets−1. ISR, insulin secretion rate.

FIG. 6.

Effect of glibenclamide on oxygen consumption rate (OCR), cytochrome c reduction, and insulin secretion rate (ISR). A: Islets were perifused with substrate-rich media as in Fig. 5A, except that glucose was 10 mmol/l throughout the experiment and 1 μmol/l glibenclamide was added for 60 min. A robust increase in insulin secretion was observed, but no significant change in oxygen consumption or cytochrome c reduction occurred. Each point represents the average of three separate experiments; the SE averaged for all data points for cytochrome c reduction was 3.3% and for oxygen consumption rate was 0.079 nmol · min−1 · 100 islets−1. B: Islets were perifused with a salt solution (KRB) as in Fig. 5B containing 10 mmol/l glucose as the only nutrient, and the effects of a pulse of 1 μmol/l glibenclamide was observed. Oxygen consumption (about one-third of that in the presence of substrate-rich media) increased by 60%, whereas little effect of glibenclamide on cytochrome c reduction was observed. Each point represents the average of four separate experiments; the SE averaged for all data points for cytochrome c reduction was 10.1% and for oxygen consumption rate was 0.095 nmol · min−1 · 100 islets−1. ISR, insulin secretion rate.

FIG. 7.

Effect of glucose and glibenclamide on calculations of ATP/ADP/Pi. The time course of ATP/ADP/Pi was calculated from Eq. 3 in the appendix and cytochrome c and oxygen data from graphs in Figs. 5 and 6. The effect of glucose on islets perifused with substrate-rich media (A) and KRB (B) and glibenclamide on islets perifused with substrate-rich RPMI (C) and KRB (D) is shown.

TABLE 1

Parameter values used to calculate ATP/ADP/Pi

Acknowledgments

This research was funded by grants from the National Institutes of Health (DK17047-26 and U42 RR16604-01).

Rats used in this study were kindly provided by Dr. Åke Lernmark.

Footnotes

    • Accepted November 29, 2003.
    • Received July 2, 2003.

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