Regulation of ATP/ADP in Pancreatic Islets

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FIG. 3.
FIG. 3.

Effect of glucose and oxygen on cytochrome c in rat islets (A and B). The concentration of glucose under normoxic conditions was switched between low and high glucose resulting in a reproducible increase and return to baseline of absorption due to cytochrome c. Pre-equilibrating the media with 95% oxygen only slightly decreased the absorption, indicating that islets were adequately oxygenated even when the media was normoxic. When the media was pre-equilibrated with 0% oxygen, the absorption increased dramatically reflecting the reduction of the cytochrome c. Spectra were obtained every minute, and the flow rate was 200 μl/min MEM.

This Article

  1. Diabetes vol. 53 no. 2 401-409