Regulation of ATP/ADP in Pancreatic Islets

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FIG. 5.
FIG. 5.

Effect of glucose on oxygen consumption rate (OCR), cytochrome c reduction, and insulin secretion rate (ISR). Islets were perifused, during which glucose was increased from 3 to 20 mmol/l for 60 min and then returned to 3 mmol/l. A: For oxygen consumption and insulin secretion, the flow rate was 50 μl/min RPMI for 300 islets, and for cytochrome c measurements, the flow rate was 200 μl/min MEM for 600 islets. The increase in glucose elicited an increase in all three parameters. Each point represents the average of three separate experiments; the SE averaged for all data points for cytochrome c reduction was 3.4% and for oxygen consumption rate was 0.1 nmol · min−1 · 100 islets−1. B: In these experiments, cytochrome c reduction, oxygen consumption, and insulin secretion were measured simultaneously. The flow rate was 100 μl/min KRB for 600 islets. Each point represents the average of six separate experiments; the SE averaged for all data points for cytochrome c reduction was 5.4% and for oxygen consumption rate was 0.6 nmol · min−1 · 100 islets−1. ISR, insulin secretion rate.

This Article

  1. Diabetes vol. 53 no. 2 401-409