Regulation of ATP/ADP in Pancreatic Islets

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FIG. 6.
FIG. 6.

Effect of glibenclamide on oxygen consumption rate (OCR), cytochrome c reduction, and insulin secretion rate (ISR). A: Islets were perifused with substrate-rich media as in Fig. 5A, except that glucose was 10 mmol/l throughout the experiment and 1 μmol/l glibenclamide was added for 60 min. A robust increase in insulin secretion was observed, but no significant change in oxygen consumption or cytochrome c reduction occurred. Each point represents the average of three separate experiments; the SE averaged for all data points for cytochrome c reduction was 3.3% and for oxygen consumption rate was 0.079 nmol · min−1 · 100 islets−1. B: Islets were perifused with a salt solution (KRB) as in Fig. 5B containing 10 mmol/l glucose as the only nutrient, and the effects of a pulse of 1 μmol/l glibenclamide was observed. Oxygen consumption (about one-third of that in the presence of substrate-rich media) increased by 60%, whereas little effect of glibenclamide on cytochrome c reduction was observed. Each point represents the average of four separate experiments; the SE averaged for all data points for cytochrome c reduction was 10.1% and for oxygen consumption rate was 0.095 nmol · min−1 · 100 islets−1. ISR, insulin secretion rate.

This Article

  1. Diabetes vol. 53 no. 2 401-409