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Effect of glucose and glibenclamide on calculations of ATP/ADP/Pi. The time course of ATP/ADP/Pi was calculated from Eq. 3 in the appendix and cytochrome c and oxygen data from graphs in Figs. 5 and 6. The effect of glucose on islets perifused with substrate-rich media (A) and KRB (B) and glibenclamide on islets perifused with substrate-rich RPMI (C) and KRB (D) is shown.