Prolonged Incubation in PUGNAc Results in Increased Protein O-Linked Glycosylation and Insulin Resistance in Rat Skeletal Muscle

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FIG. 3.
FIG. 3.

O-linked glycosylation of muscle proteins following 19 h of incubation of paired skeletal muscles with or without 100 μmol/l PUGNAc. A: Muscle homogenates were subjected to 8% SDS-PAGE. Subsequent protein blots were probed with anti-O-GlcNAc and visualized following enhanced chemiluminescence. Relative molecular weight standards are denoted. B: Protein bands were quantitated by densitometry, and values for PUGNAc-treated samples are expressed relative to the mean value for the corresponding protein band in muscles incubated without PUGNAc (mean ± SE; n = 6 per group). *Significant difference for paired muscles with PUGNAc versus without PUGNAc (P < 0.005).

This Article

  1. Diabetes vol. 53 no. 4 921-930