Phosphorylation status of Akt(T308) following 19 h incubation of muscle with or without 100 μmol/l PUGNAc. A: Dose response for insulin in muscles incubated without PUGNAc at basal (no insulin), 0.6, or 12 nmol/l insulin during the
final 75 min of incubation (n = 4 per group). Paired muscles incubated with or without PUGNAc for 19 h and with 0.6 nmol/l insulin (B) during the final 75 min of incubation (n = 11 per group) or 12 nmol/l insulin (C) during the final 75 min of incubation (n = 12 per group). Bands corresponding to P-Akt(T308) were quantitated by densitometry and expressed as the mean ± SE. *Basal
versus 0.6 nmol/l insulin (P < 0.005); **basal versus 12 nmol/l insulin (P < 0.001) and 0.6 vs. 12 nmol/l insulin (P < 0.001).