Deficiency of Subsarcolemmal Mitochondria in Obesity and Type 2 Diabetes

TABLE 1

Sequences of amplification primers and probes

Primer Sequence
    mtDNA-forward 5′–GAC GCC TCA ACC GCC TTT–3′
    mtDNA-reverse 5′–GCG GAT GAT TCA GCC ATA ATT TA–3′
    β-Globin-forward 5′–TGA AGG CTC ATG GCA AGA AA–3′
    β-Globin-reverse 5′–AAA GGT GCC CTT GAG GTT GTC–3′
Probe Sequence
    mtDNA probe 5′–6FAM-CAT CAA TCG CCC ACA TCA CTC GAG AC-TAMRA–3′
    β-Globin probe 5′–6FAM-CCA GGC CAT CAC TAA AGG CAC CGA-TAMRA–3′

This Article

  1. Diabetes vol. 54 no. 1 8-14