Glucose Transporters in Human Renal Proximal Tubular Cells Isolated From the Urine of Patients With Non–Insulin-Dependent Diabetes


Expression of relevant cellular markers in human exfoliated primary cells and HEPTECs

Cellular marker mRNA* Protein* Cell type
Cytokeratin-7 ND +++ High in distal cells and bladder cells and low in proximal cells
Aquaporin-2 ND ++ Collecting duct
ENaCα +++ +++ Collecting duct and distal cells
ENaCβ/γ ++ ND Collecting duct and distal cells
PPARγ/α/δ +++ PPARγ only Collecting duct and distal cells/proximal cells
NaCl cotransporter +++ ND Proximal cells/connecting tubules
Aquaporin-2 ND +++ Collecting duct
L1-CAM (cell adhesion molecule) ND +++ Collecting duct
Aminopeptidase-N (CD13) ND +++ Proximal cells
Sodium-glucose transporters (SGLT2, GLUT1, and GLUT2) +++ +++ Proximal cells
Alkaline phosphatase ND +++ Proximal cells
Uroplakin III ND +++ Bladder cells
Mineralocortcoid receptor +++ ND Connecting tubules/distal cells/collecting duct
  • Expression of cellular markers of human primary exfoliated cells: 1) bladder/urethra (uroplakin III/cytokeratin 7), 2) proximal tubular cells (CD13 aminopepetidase-N/SGLT2, 3) renal distal tubular/collecting duct cells (aldosterone receptor, cytokeratin-7, aquaporin-2, and ENaCα) by PCR and/or immunoblotting. Marker level of presence (+ + +).

  • *

    * mRNA (Taqman) and proteins were measured and expressed as relative abundance to gapdh. +, weak expression; ++ high expression; +++ very high expression; ND, not been done.

This Article

  1. Diabetes vol. 54 no. 12 3427-3434