Gene Transfer of Constitutively Active Akt Markedly Improves Human Islet Transplant Outcomes in Diabetic Severe Combined Immunodeficient Mice

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIG. 5.
FIG. 5.

Long-term function of Adv-LacZ- and Adv-CA-Akt–transduced islet grafts. Blood glucose in three of six randomly selected mice transplanted with 1,500 Adv-LacZ–or 1,500 Adv-CA-Akt–transduced IEQs was measured for a period of 6 months. The posttransplant blood glucose values in mice transplanted with 1,500 Adv-CA-Akt–transduced IEQs were significantly (P < 0.05) lower for the entire 6-month period than the blood glucose values obtained after the transplant of 1,500 Adv-LacZ–transduced human IEQs. In the group of mice transplanted with 1,500 Adv-LacZ IEQs, one hyperglycemic transplanted mouse died at 175 days after the transplant. At day 180, UNX was performed in mice transplanted with Adv-CA-Akt–transduced islets, and the blood glucose returned immediately to pretransplant diabetic levels. Results are means ± SE.

This Article

  1. Diabetes vol. 54 no. 6 1664-1675