Gene Transfer of Constitutively Active Akt Markedly Improves Human Islet Transplant Outcomes in Diabetic Severe Combined Immunodeficient Mice

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FIG. 6.
FIG. 6.

Immunohistochemical detection of hemagglutinin and different islet and ductal cell markers in kidney sections containing Adv-CA-Akt grafts. Kidney sections from mice transplanted with 1,500 Adv-LacZ or 1,500 Adv-CA-Akt human IEQs obtained 6 months after the transplant were stained for insulin as described in research design and methods. The transplant could not be identified in any of the Adv-LacZ kidneys despite sectioning throughout the entire organ. In contrast, grafts were large and easily identified in all Adv-CA-Akt kidneys. Representative photomicrographs of sections from Adv-CA-Akt kidneys are shown displaying positive staining for insulin (A, E, and F), CK-19 (B), glucagon (C), and somatostatin (D). Notice the presence of insulin-positive cells inside of ductal structures in the grafts (arrows in E and F), suggesting β-cell neogenesis. G and H: Kidney sections from mice transplanted with 1,500 Adv-CA-Akt human IEQs obtained 6 months after the transplant were stained for hemagglutinin (red), insulin (green), and Hoestch 33258 (blue) as described in research design and methods. Arrows indicate hemagglutinin-positive cells. I and J: No hemagglutinin staining was observed in graft sections obtained at 56 days after the transplant of 4,000 uninfected IEQs.

This Article

  1. Diabetes vol. 54 no. 6 1664-1675