Inhibition of Microsomal Triglyceride Transfer Protein Expression and Apolipoprotein B100 Secretion by the Citrus Flavonoid Naringenin and by Insulin Involves Activation of the Mitogen-Activated Protein Kinase Pathway in Hepatocytes

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FIG. 4.
FIG. 4.

Naringenin and insulin decrease apoB100 secretion from hepatocytes via activation of the MAPKerk pathway. HepG2 cells in LPDS media were preincubated (30 min) ± UO126 (10 μmol/l) or UO124 (10 μmol/l) (A), or ± SB203580 (10 μmol/l) or SB202474 (10 μmol/l) (B) followed by a 23.5-h incubation ± naringenin (100 μmol/l) or insulin (100 nmol/l). Media apoB100 was measured by immunoblotting and expressed as percent of control. Values are means ± SE for at least four experiments. *P < 0.05, **P < 0.01 compared with control; †P < 0.05 compared with naringenin or insulin.

This Article

  1. Diabetes vol. 54 no. 6 1676-1683