Combined inhibition of PI 3-kinase and MEK1/2 reverses the naringenin- and insulin-mediated decrease in apoB100 secretion.
HepG2 cells in LPDS media were preincubated for 30 min ± wortmannin (1 μmol/l) and/or UO126 (10 μmol/l), followed by a further
23.5-h incubation ± naringenin (100 μmol/l) or insulin (100 nmol/l). Media apoB100 was measured by immunoblotting and expressed
as percent of control. Values are means ± SE for at least three experiments. *P < 0.05, **P < 0.01 compared with control; †P < 0.05, ‡P < 0.01 compared with naringenin or insulin; §P < 0.05 compared with wortmannin plus UO126 alone; ¶P < 0.05 compared with wortmannin plus insulin.