Inhibition of Microsomal Triglyceride Transfer Protein Expression and Apolipoprotein B100 Secretion by the Citrus Flavonoid Naringenin and by Insulin Involves Activation of the Mitogen-Activated Protein Kinase Pathway in Hepatocytes

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FIG. 5.
FIG. 5.

Combined inhibition of PI 3-kinase and MEK1/2 reverses the naringenin- and insulin-mediated decrease in apoB100 secretion. HepG2 cells in LPDS media were preincubated for 30 min ± wortmannin (1 μmol/l) and/or UO126 (10 μmol/l), followed by a further 23.5-h incubation ± naringenin (100 μmol/l) or insulin (100 nmol/l). Media apoB100 was measured by immunoblotting and expressed as percent of control. Values are means ± SE for at least three experiments. *P < 0.05, **P < 0.01 compared with control; †P < 0.05, ‡P < 0.01 compared with naringenin or insulin; §P < 0.05 compared with wortmannin plus UO126 alone; ¶P < 0.05 compared with wortmannin plus insulin.

This Article

  1. Diabetes vol. 54 no. 6 1676-1683