Effect of glucose and insulin on the mRNA level of GLUT2 and SREBPs in primary cultured hepatocytes. A: Hepatocytes isolated from mice were plated for 5 h and cultured for a further 16 h in the presence or absence of insulin
(100 nmol/l) and glucose (25 mmol/l). Total RNA was extracted from each treatment group as described in research design and methods. B–E: Quantification of hepatic mRNA for GLUT2, SREBP-1c, SREBP-1a, and SREBP-2. Total RNA (3 μg) used in reverse transcription
experiments was subjected to real-time PCR. The data were quantified as described in research design and methods. All mRNA levels were normalized to that of α-tubulin. The results are the means ± SD of three independent experiments with
triplicate mea-surements. B: *P < 0.001 for the untreated vs. glucose-treated group; C: *P < 0.005 for the untreated vs. insulin-treated group, #P < 0.05 for the untreated vs. glucose-treated group, and †P < 0.001 for the untreated vs. glucose/insulin-treated group.