Effect of SREBPs on GLUT2 promoter activity in the Alexander cell lines. pmGT2-1112 promoter reporter was cotransfected with
SREBP-1c (100 ng) or SREBP-2 (100 ng) to Alexander cell lines, which were plated at a density of 2 × 105 cells/35-mm dish. Transient transfection, luciferase assays, and measurement of total protein concentration of the lysates
were performed as described in research design and methods. Luciferase activities were normalized by the amount of total protein. Each transfection was performed in triplicate and
repeated three to five times. *P < 0.001 for the untreated vs. SREBP-1–transfected group; #P < 0.05 for the untreated vs. SREBP-2–transfected group.