Glucose stimulation of GLUT2 gene expression. A and B: Primary hepatocytes, which were fasted for 24 h, were maintained in media containing the indicated concentration of glucose
for 16 h. GLUT2 mRNA and protein levels were quantified by Northern (A) and Western (B) blot analyses. The blots are representative of two different experiments. C: Effect of SREBP-1c DN on glucose-stimulated GLUT2 expression. Adenovirus containing SREBP-1c or null adenovirus (adeno-GFP)
was transduced into primary hepatocytes at a titer of 5 pfu per cell for 2 h at 37°C. Cells were incubated in the presence
(25 mmol/l) or absence of glucose for 24 h, and RNA was harvested from the cells. The data were quantified as described in
research design and methods. The mRNA level was normalized to that of GAPDH. The results are the means ± SD of three independent experiments with triplicate
measurements. *P < 0.005 for Adeno-GFP vs. glucose; #P < 0.05 for the glucose vs. glucose plus SREBP-1c DN.