Glucose-Stimulated Upregulation of GLUT2 Gene Is Mediated by Sterol Response Element–Binding Protein-1c in the Hepatocytes
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ChIP assay. A: Effect of glucose or insulin on the SREBP-1 binding to putative GLUT2-SRE. Chromatin was precipitated using a SREBP-1 antibody
from primary hepatocytes, and the GLUT2 promoter region was amplified by PCR. The amount of chromosomal DNA used in immunoprecipitation
between groups was normalized by input chromatin (100th of chromosomal DNA used for immunoprecipitation). B: Effect of glucose concentrations on the SREBP-1 binding to putative GLUT2-SRE. Glucose concentrations are shown on the top.
Detailed PCR conditions and methods of sample treatment are described in research design and methods.