Insulin and Leptin Resistance With Hyperleptinemia in Mice Lacking Androgen Receptor

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FIG. 2.
FIG. 2.

AR−/y mice are insulin resistant and glucose intolerant. A: GTT (oral bolus 2 mg/g body wt) of 35-week-old mice after 14-h fast. B: area under the curve analysis of GTT. C: ITT (1 unit/kg body wt i.p.) of 25-week-old mice. D: ITT (1 unit/kg body wt i.p.) of 35-week-old mice. Values are the means ± SE from 5–6 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001, AR−/y vs. male wild-type mice. E: In vivo PI3K activity was measured in phosphotyrosine immunoprecipitates prepared from skeletal muscles, adipose tissues, and livers from 14 h–fasted 35-week-old mice. Tissues were collected 3 min after intraperitoneal injection of insulin or isotonic saline. The results were quantified by phosphoimaging. Values are representative of three mice from each group. **P < 0.01, AR−/y with insulin vs. wild type with insulin. F: Skeletal muscles and livers were removed from 35-week-old ad libitum–fed mice. Values of each group were converted into milligrams of triglyceride per gram of tissue (wet weight) after comparison with a glycerol standard. Values are the means ± SE from five mice per group. ***P < 0.001, AR−/y vs. wild-type mice. PI(3)P, phosphatidylinositol-3-phosphate; w/, with; w/o, without; WT, wild type.

This Article

  1. Diabetes vol. 54 no. 6 1717-1725