Insulin and Leptin Resistance With Hyperleptinemia in Mice Lacking Androgen Receptor

TABLE 3

Effects of dihydrotestosterone treatment on serum hormones and metabolic parameters in 20-week-old study groups

Male wild-type 20-week-old (12–20 weeks) mice
AR−/y 20-week-old (12–20 weeks) mice
Sham Placebo Sham Placebo Dihydrotestosterone
Mice (n) 4 4 4 4 4
Insulin resistance index 945.6 ± 22.6 1,095.1 ± 73.5 5,540.4 ± 182.7* 4,473.3 ± 255.1* 4,478.4 ± 394.4*
IGTT AUC insulin (ng · ml−1 · min−1) 44.50 ± 8.84 48.30 ± 6.55 178.55 ± 12.15* 152.16 ± 9.39* 136.40 ± 12.7*
IGTT AUC glucose (g · dl−1 · min−1) 21.25 ± 0.50 22.67 ± 1.52 31.61 ± 0.92 29.39 ± 1.67* 32.83 ± 2.89
Serum insulin (ng/ml) 0.25 ± 0.06 0.29 ± 0.02 0.61 ± 0.01 0.71 ± 0.10 0.68 ± 0.04
Blood glucose (mmol/l) 6.16 ± 0.09 5.99 ± 0.36 8.02 ± 0.53 8.00 ± 0.09 8.69 ± 0.49
Leptin (ng/ml) 0.88 ± 0.17 0.69 ± 0.09 7.32 ± 0.32 7.82 ± 1.03 8.12 ± 0.82
Cholesterol (mmol/l) 1.59 ± 0.07 1.73 ± 0.30 1.62 ± 0.11 1.68 ± 0.01 1.72 ± 0.16
Nonesterified fatty acid (mEq/l) 0.18 ± 0.03 0.18 ± 0.02 0.33 ± 0.01* 0.36 ± 0.03* 0.30 ± 0.05
Total triglycerides (mmol/l) 0.15 ± 0.02 0.17 ± 0.04 0.29 ± 0.08 0.28 ± 0.04 0.26 ± 0.02
Adiposity index (%) 1.34 ± 0.03 1.15 ± 0.06 1.72 ± 0.05 1.71 ± 0.09 1.74 ± 0.02
Androstenedione (ng/ml) 0.58 ± 0.11 0.62 ± 0.07 0.64 ± 0.05 0.59 ± 0.14 0.63 ± 0.09
Estradiol (pg/ml) 55.97 ± 5.48 54.65 ± 4.37 57.06 ± 1.72 59.82 ± 1.60 54.38 ± 1.41
Dihydrotestosterone (ng/ml) 0.61 ± 0.05 0.58 ± 0.07 0.09 ± 0.03 0.12 ± 0.05 0.73 ± 0.09
  • Data are the means ± SE After a 14-h fast, animals were killed. The adiposity index was the ratio of epididymal adipose tissue to body weight for mice. The insulin resistance index is the product of glucose and insulin areas under the curve. Parentheses indicate the period of pellet implantation.

  • *

    * P < 0.05,

  • P < 0.01, and

  • P < 0.001, vs. sham male wild-type mice. AUC, area under the curve; IGTT, intraperitoneal GTT.

This Article

  1. Diabetes vol. 54 no. 6 1717-1725