A Human-Specific Role of Cell Death-Inducing DFFA (DNA Fragmentation Factor-α)-Like Effector A (CIDEA) in Adipocyte Lipolysis and Obesity

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FIG. 3.
FIG. 3.

Cross-talk between CIDEA and TNF-α in human preadipocytes. A: Ratios of CIDEA to GAPDH mRNA levels in differentiated preadipocytes after 48 h of incubation without (□) or with (▪) TNF-α (n = 8). B: Ratios of CIDEA to GAPDH mRNA in differentiated preadipocytes after 48 h of incubation without (□) or with (▪) TNF-α alone or with TNF-α and the indicated dilutions of the specific JNK inhibitor SP600125 (SP) (▒). The concentration 1:1 corresponds to 20 μmol/l. The figure shows one of three representative experiments. C: Ratios of CIDEA to GAPDH mRNA levels and glycerol release in preadipocytes after 24 h of RNA interference (n = 6). P < 0.05, Spearman’s correlation. D: Ratios of CIDEA to GAPDH mRNA levels and TNF-α protein secretion after 24 h of incubation with different amounts of CIDEA siRNA (n = 8). P < 0.01, Spearman’s correlation. *P < 0.05, Wilcoxon’s signed-rank test.

This Article

  1. Diabetes vol. 54 no. 6 1726-1734