Effects of CIDEA depletion on TNF-α mRNA levels and different TNF-α targets. A: Ratios of CIDEA and TNF-α to GAPDH mRNA levels in preadipocytes after 24 h of incubation with different amounts of CIDEA siRNA (n = 8). P = 0.29, Spearman’s correlation. B: CIDEA, perilipin (PLIN), and GAPDH mRNA levels in preadipocytes after 24 h of RNA interference (n = 4). *P < 0.05, Wilcoxon’s signed-rank test. C and D: Ratios of CIDEA and MCP-1 to GAPDH mRNA levels (C) and MCP-1 protein secretion into cell media (D) after 24 h of RNA interference (n = 6). P = 0.53 and P = 0.38 for C and D, respectively; Spearman’s correlation.