Prevention of Type 1 Diabetes with Major Histocompatibility Complex–Compatible and Nonmarrow Ablative Hematopoietic Stem Cell Transplants

TABLE 2

Prevalence of diabetes and insulitis in transplanted NOD mice

Transplanted mice Recipient sex Pancreas histology
Hyperglycemia
No. of islets observed Histology score (%)
0 1 2 3
C57BL/6.H-2g7 WBM → NOD 1 F 11 46 18 9 27 No
C57BL/6.H-2g7 WBM → NOD 2 F 14 43 22 21 14 No
C57BL/6.H-2g7 HSC → NOD 1 F 7 86 0 14 0 No
C57BL/6.H-2g7 HSC → NOD 2 F 8 87 13 0 0 No
NOD control 1 F 13 0 0 15 85 No
NOD control 2 F 22 23 9 36 32 No
C57BL/6.H-2g7 control 1 F 13 100 0 0 0 No
C57BL/6.H-2g7 control 2 F 16 100 0 0 0 No
C57BL/6.H-2g7 WBM → NOD.SCID M 5 100 0 0 0 No
  • Islet cell infiltration: NOD mice were conditioned with 950 cGy and transplanted with HSCs or bone marrow from B6.H-2g7 mice were killed 6 months posttransplantation. Eight-week-old unmanipulated B6.H-2g7 and NOD mice are included in the analysis. Adjacent sections of pancreata were analyzed, and islet infiltrates were scored according to a grading system described by Wicker et al. (54). Histology score: grade 0, no infiltration; grade 1, perivascular/periductular or perinsular infiltration, but not penetrating; grade 2, mild leukocytic islet mass infiltration; grade 3, severe infiltration, islet mass destruction. WBM, whole bone marrow.

This Article

  1. Diabetes vol. 54 no. 6 1770-1779